Three distinct domains contribute to nuclear transport of murine Foxp3.

PloS One
Wayne W Hancock, Engin Ozkaynak

Abstract

Foxp3, a 47-kDa transcription factor, is necessary for the function of CD4+CD25+ regulatory T cells (Tregs), with an essential role in the control of self-reactive T cells and in preventing autoimmunity. Activation of Tregs by TCR engagement results in upregulation of Foxp3 expression, followed by its rapid nuclear transport and binding to chromatin. Here, we identify three distinct Foxp3 domains that contribute to nuclear transport. The first domain (Domain 1) comprises the C-terminal 12 amino acids. The second domain (Domain 2) is located immediately N-terminal to the forkhead domain (FHD), recently reported to be a binding site for the runt-related transcription factor 1/acute myeloid leukemia 1 (Runx1/AML1). The third domain (Domain 3) is located within the N-terminal first 51 amino acids. Unlike the known nuclear localization signals (NLSs), none of these three regions are rich in basic residues and do not bear any similarity to known monopartite or bipartite NLSs that have one or more clusters of basic amino acids. The basic arginine-lysine-lysine-arginine (RKKR) sequence, located 12-aa from the C-terminal end of Foxp3 was previously reported to be a nuclear localization signal (NLS) for several proteins, including for a ...Continue Reading

References

Nov 1, 1987·Molecular and Cellular Biology·R B MorelandL M Hereford
Dec 5, 1998·Journal of Molecular Biology·I Nilsson, G von Heijne
Jul 25, 2000·Biochemical and Biophysical Research Communications·Y ZengH Kanaide
Mar 4, 2003·Nature Immunology·Jason D FontenotAlexander Y Rudensky
Mar 16, 2004·The Journal of Biological Chemistry·Anne StefanssonStaffan Johansson
Feb 17, 2005·Molecular and Cellular Biology·Yoshikazu MikamiTatsuo Fukagawa
Mar 26, 2005·Proceedings of the National Academy of Sciences of the United States of America·Estelle BettelliMohamed Oukka
Apr 1, 2006·The Journal of Biological Chemistry·Joseph H SongKirill A Martemyanov
Aug 22, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jared E LopesSteven F Ziegler
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Bin LiMark I Greene
Mar 23, 2007·Nature·Masahiro OnoShimon Sakaguchi
Nov 7, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Sarah E AllanMegan K Levings
Mar 21, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jianguang DuSteven F Ziegler
Jan 2, 2009·The Journal of Biological Chemistry·Edwin F de ZoetenEngin Ozkaynak

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Citations

Jul 27, 2012·Cancer Metastasis Reviews·Stephen DouglassJohn A Kirby
Nov 30, 2011·Immunology and Cell Biology·Hongtao ZhangMark I Greene
Jul 28, 2013·International Reviews of Immunology·Veerle Fleskens, Ruben van Boxtel
Mar 18, 2015·International Journal of Oncology·Yu-Jie LiangGui-Qing Liao
Jul 30, 2011·Current Opinion in Immunology·Ulf H BeierWayne W Hancock
Apr 22, 2011·Trends in Genetics : TIG·Bérénice A BenayounReiner A Veitia
Jan 20, 2011·BJU International·Malin E WinerdalOla Winqvist
Feb 17, 2015·Scandinavian Journal of Immunology·R ElhageD Klatzmann
Jul 21, 2012·PloS One·Sebastian KönigLothar Jänsch
Apr 15, 2011·Molecular Oncology·Margaret RedpathAlan Spatz
Dec 23, 2017·Proceedings of the National Academy of Sciences of the United States of America·Ho-Keun KwonChristophe Benoist
Aug 2, 2017·Nature Reviews. Immunology·Ling LuFan Pan
Nov 5, 2019·Frontiers in Immunology·Guoping DengMark I Greene
Aug 7, 2019·Clinical and Experimental Immunology·G DengM I Greene
Jun 18, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Timothy J SadlonSimon C Barry
May 4, 2013·Journal of Genetics·Julie Massayo Maeda OdaMaria Angelica Ehara Watanabe
Feb 3, 2021·The FEBS Journal·Biaolong DengBin Li

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Methods Mentioned

BETA
acetylation
nuclear translocation
nuclear
PMA

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