PMID: 9171375May 15, 1997Paper

Three human RNA polymerase III-specific subunits form a subcomplex with a selective function in specific transcription initiation

Genes & Development
Z Wang, R G Roeder

Abstract

Transcription by RNA polymerase III involves recruitment of the polymerase by template-bound accessory factors, followed by initiation, elongation, and termination steps. An immunopurification approach has been used to demonstrate that human RNA Pol III is composed of 16 subunits, some of which are apparently modified in HeLa cells. Partial denaturing conditions and sucrose gradient sedimentation at high salt result in the dissociation of a subcomplex that includes hRPC32, hRPC39, and hRPC62. Cognate cDNAs were isolated and shown to encode three subunits that are specific to RNA Pol III and homologous to three yeast subunits. The human RNA Pol III core lacking the subcomplex functions in transcription elongation and termination following nonspecific initiation on a tailed template, but fails to show promoter-dependent transcription initiation in conjunction with accessory factors. The capability for specific transcription initiation can be restored either by the natural subcomplex or by a stable subcomplex composed of recombinant hRPC32, hRPC39, and hRPC62 polypeptides. One component (hRPC39) of this subcomplex interacts physically with both hTBP and hTFIIIB90, two subunits of human RNA Pol III transcription initiation factor I...Continue Reading

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Citations

Mar 2, 2011·Nature Structural & Molecular Biology·Stéphane LefèvreSébastien Fribourg
Feb 16, 2010·Proceedings of the National Academy of Sciences of the United States of America·Valérie HaurieMartin Teichmann
Nov 8, 2012·Proceedings of the National Academy of Sciences of the United States of America·Chih-Chien WuHung-Ta Chen
Jul 8, 1999·Proceedings of the National Academy of Sciences of the United States of America·A FloresM Werner
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Aug 2, 2000·The EMBO Journal·V McCullochS M Lobo-Ruppert
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Mar 8, 2012·Nucleic Acids Research·Stefan JennebachPatrick Cramer
Dec 3, 2004·Genes & Development·Ping HuNouria Hernandez
Jun 17, 2006·Genes & Development·Robert ReinerNayef Jarrous
Oct 11, 2013·Genome Research·Marianne RenaudNouria Hernandez
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Oct 5, 2010·Cell Cycle·Hélène Dumay-OdelotMartin Teichmann

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