PMID: 11336788May 5, 2001Paper

Three kinds of currents in the canine betaine-GABA transporter BGT-1 expressed in Xenopus laevis oocytes

Biochimica Et Biophysica Acta
Greta ForlaniAntonio Peres

Abstract

The cloned canine betaine-GABA cotransporter BGT-1 has been heterologously expressed in Xenopus laevis oocytes in order to characterize its electrophysiological properties. Voltage-clamp experiments on transfected oocytes reveal the presence of three types of membrane current which are absent in non-injected oocytes: (i) an organic substrate-independent current (uncoupled current); (ii) a transport-associated current, seen upon addition of betaine or GABA; (iii) presteady-state currents induced by voltage changes. The three kinds of current are analogous to those reported in structurally similar cotransporters. The transport-associated current is strictly dependent on the presence of Na(+). The good correlation between the amount of charge underlying the presteady-state currents and the transport-associated current indicates that both processes are due to the activity of the transporter.

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Citations

Jun 29, 2005·The Journal of Membrane Biology·M H KarakossianS Eskandari
Nov 13, 2007·The Journal of Membrane Biology·Albert L GonzalesSepehr Eskandari
May 9, 2003·American Journal of Physiology. Cell Physiology·V Franca SacchiAntonio Peres
Jul 15, 2011·Pharmacological Reviews·Anders S KristensenUlrik Gether
Jul 13, 2007·American Journal of Physiology. Cell Physiology·M CastagnaV F Sacchi

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