Three Lactobacillus strains from healthy infant stool inhibit enterotoxigenic Escherichia coli grown in vitro

Anaerobe
Cheng-Chih TsaiYou-Miin Hsieh

Abstract

Enterotoxigenic Escherichia coli (ETEC) are a major cause of sporadic diarrhea disease in humans, affecting mainly infants in developing countries and travelers from industrialized countries visiting tropical or subtropical areas. In this study, we screen the antagonistic activity by inoculating wells among ETEC agar cultures to assess inhibition zones created by the lactobacilli spent culture supernatant (SCS) from healthy infant stool. Only three isolates possessed antagonistic activity, acid and bile tolerance and could adhere to the cultured human intestinal C2BBel (Caco-2) cell line. Isolate identification using API 50CHL strips showed that they belonged to different Lactobacillus species, i.e., Lactobacillus acidophilus RY2, Lactobacillus salivarius MM1 and Lactobacillus paracasei En4. The SCS still had an inhibitory effect on ETEC after heating (100 degrees C, 15 min). The lactate dehydrogenase treatment or the pH of SCS was adjusted to neutral (pH 7.2) to reduce the SCS inhibitory effect. Antimicrobial activity was performed by incubating the lactic acid bacteria (LAB)-SCS with ETEC suspension. After 4h of co-culture, ETEC growth was inhibited. This study suggests that L. acidophilus RY2, L. salivarius MM1 and L. paraca...Continue Reading

References

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Citations

Feb 13, 2013·The British Journal of Nutrition·Luis FontanaAngel Gil
Oct 6, 2012·Annals of Nutrition & Metabolism·Miriam Bermudez-BritoAngel Gil
Nov 20, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Cheng-Chih TsaiYung-Sheng Lin
Feb 13, 2016·Jundishapur Journal of Microbiology·Abolfazl DavoodabadiMaryam Tajabadi Ebrahimi
Nov 3, 2015·Environmental Microbiology·Lucia Gonzales-Siles, Åsa Sjöling
Dec 29, 2009·Wiener klinische Wochenschrift·Angelika Wagner, Ursula Wiedermann
Nov 3, 2016·Pediatrics International : Official Journal of the Japan Pediatric Society·Yoon Hee ShimJung Won Lee
Jan 1, 2014·The Journal of General and Applied Microbiology·Nazila AazamiMahdi Goudarzvand
Jan 10, 2020·Current Pharmaceutical Biotechnology·Huey-Chun HuangTsong-Min Chang
Oct 4, 2020·Microorganisms·Anastasia MantziariJeadran Nevardo Malagón-Rojas
Dec 23, 2019·Journal of the International Society of Sports Nutrition·Ralf JägerJose Antonio

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