Three manual immunoassays for the rapid detection of hepatitis B surface antigen

Transactions of the Royal Society of Tropical Medicine and Hygiene
G J FinnyG Sridharan

Abstract

We have evaluated 3 commercial test kits for the detection of hepatitis B surface antigen (HBsAg) which do not require elaborate instrumentation and are relatively inexpensive. These included the Immunocomb-II (Orgenics, Israel), Austragen latex agglutination (Span, India) and Daina Screen (Abbott, USA) tests. A panel of 98 sera tested by IMx HBsAg assay (Abbott, USA) were used to evaluate the tests. Sensitivities of 96.6%, 86.2% and 96.6%, and specificities of 95.7%, 94.2% and 100%, respectively, were obtained. We therefore recommend the Immunocomb-II or Daina Screen tests for HBsAg detection in small laboratories where facilities to conduct automated enzyme-linked immunosorbent assays are not available.

References

Nov 26, 1977·British Medical Journal·A K ChaudhuriC J Burrell
Feb 1, 1977·American Journal of Epidemiology·R P BeasleyW Szmuness
Sep 1, 1989·JAMA : the Journal of the American Medical Association·M J AlterH S Margolis
Mar 1, 1974·Archives of Internal Medicine·J B Smith, L F Barker
Aug 1, 1993·Immunological Reviews·B C Fellström, E Larsson

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Citations

Jun 26, 2003·Journal of Viral Hepatitis·D T-Y LauJ H Hoofnagle

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