Three novel point mutations of the CYP21 gene detected in classical forms of congenital adrenal hyperplasia due to 21-hydroxylase deficiency

Experimental and Clinical Endocrinology & Diabetes : Official Journal, German Society of Endocrinology [and] German Diabetes Association
Nils KroneWolfgang G Sippell

Abstract

Congenital adrenal hyperplasia (CAH) [OMIM 201 910] is a group of autosomal recessive disorders most commonly due to 21-hydroxylase deficiency and presenting with a wide range of clinical manifestations. A limited number of inactivating pseudogene-derived mutations account for the majority of 21-hydroxylase gene ( CYP21) mutations, additional rare mutations can be found in single families and small populations. We found three novel CYP21 mutations in CAH patients suffering from the classical form of the disease, of which one is a frameshift mutation (1353-1354insA) leading to a premature termination codon (K277K, Q228A...E294X), one results in a premature stop codon (2551C>T, R444X), and one is a missense mutation (2609T>C; P463L). The frameshift and premature stop mutations can be predicted to result in a CYP21 protein without any residual enzyme activity. To determine the functional consequences of the P463L mutation, the IN VITRO enzyme activity was studied in COS-7 cells and revealed a reduced 21-hydroxylase activity of 2.6+/-0.8 (SD)% for the conversion of 17-hydroxyprogesterone (17OHP) to 11-deoxycortisol and of 3.0+/-0.5 % for the conversion of progesterone to 11-deoxycorticosterone (DOC). We conclude that functional ana...Continue Reading

Citations

Oct 9, 2012·Journal of Paediatrics and Child Health·Vinutha B ShettyElizabeth A Davis
Oct 12, 2010·The Journal of Clinical Endocrinology and Metabolism·Gabriela P FinkielstainDeborah P Merke
Jan 11, 2005·Molecular Genetics and Metabolism·Hsien-Hsiung Lee
Oct 11, 2016·International Journal of Endocrinology·Débora de Paula MichelattoSvetlana Lajic
Apr 3, 2008·The Journal of Clinical Endocrinology and Metabolism·F C SoardiM P de Mello
Dec 8, 2015·Journal of Pediatric Endocrinology & Metabolism : JPEM·Bryan A GhannyRoja Motaghedi
Jul 4, 2020·European Journal of Human Genetics : EJHG·Sabina Baumgartner-ParzerWolfgang Hoeppner
Jul 25, 2019·Frontiers in Endocrinology·Duarte PignatelliDjuro Macut

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