Three novel proteins co-localise with polyhydroxybutyrate (PHB) granules in Rhodospirillum rubrum S1

Microbiology
Tanja NarancicKevin E O'Connor

Abstract

Polyhydroxybutyrate (PHB), a biodegradable polymer accumulated by bacteria is deposited intracellularly in the form of inclusion bodies often called granules. The granules are supramolecular complexes harbouring a varied number of proteins on their surface, which have specific but incompletely characterised functions. By comparison with other organisms that produce biodegradable polymers, only two phasins have been described to date for Rhodosprillum rubrum, raising the possibility that more await discovery. Using a comparative proteomics strategy to compare the granules of wild-type R. rubrum with a PHB-negative mutant housing artificial PHB granules, we identified four potential PHB granules' associated proteins. These were: Q2RSI4, an uncharacterised protein; Q2RWU9, annotated as an extracellular solute-binding protein; Q2RQL4, annotated as basic membrane lipoprotein; and Q2RQ51, annotated as glucose-6-phosphate isomerase. In silico analysis revealed that Q2RSI4 harbours a Phasin_2 family domain and shares low identity with a single-strand DNA-binding protein from Sphaerochaeta coccoides. Fluorescence microscopy found that three proteins Q2RSI4, Q2EWU9 and Q2RQL4 co-localised with PHB granules. This work adds three potential...Continue Reading

References

Sep 27, 2000·Journal of Bacteriology·R HandrickD Jendrossek
Feb 1, 1964·The Journal of Cell Biology·E S BOATMAN
May 8, 2007·Nucleic Acids Research·Lukas KällErik L L Sonnhammer
Jul 30, 2008·Bioinformatics·Romain GautierGuillaume Drin
Mar 10, 2009·Journal of Bacteriology·Dieter Jendrossek
Jan 25, 2011·Journal of Proteome Research·Jürgen CoxMatthias Mann
Sep 3, 2011·Standards in Genomic Sciences·A Christine MunkDavid C Schwartz
Nov 20, 2012·BMC Microbiology·Andreas WahlDieter Jendrossek
Oct 5, 2014·Applied and Environmental Microbiology·Jessica Eggers, Alexander Steinbüchel
Feb 28, 2015·Nanomedicine : Nanotechnology, Biology, and Medicine·Nina Dinjaski, M Auxiliadora Prieto
Apr 18, 2015·Nucleic Acids Research·Alexey DrozdetskiyGeoffrey J Barton
May 26, 2016·Scientific Reports·Stephanie BresanDieter Jendrossek
Jun 28, 2016·Nature Methods·Stefka TyanovaJürgen Cox
Apr 21, 2017·Microbial Biotechnology·Beatriz Maestro, Jesús M Sanz

❮ Previous
Next ❯

Citations

Jul 12, 2020·Applied and Environmental Microbiology·Guillaume Bayon-VicenteBaptiste Leroy
Jul 6, 2019·Archives of Microbiology·Tatiana KulakovskayaMikhail Vainshtein
Oct 30, 2020·FEMS Microbiology Reviews·Marcelo Müller-SantosAnna Maria Pirttilä
May 7, 2021·FEMS Microbiology Reviews·Marcelo Müller-SantosAnna Maria Pirttilä

❮ Previous
Next ❯

Related Concepts

Related Feeds

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.