Abstract
We report on the cloning and sequencing of two Tranosema rostrale ichnovirus (TrIV) genes, and assess their relatedness to TrV1, the gene encoding the most abundant TrIV transcript in last-instar Choristoneura fumiferana larvae parasitized by T. rostrale. One of the two newly isolated genes, TrV2, features an organization similar to that of TrV1, with one intron flanked by two exons; it encodes a 102 amino acid protein showing 79% similarity to TrV1. The third gene, TrV4, encodes a larger protein (143 aa) displaying similarity to the other two only over the first approximately 50 amino acid residues of its sequence; the remaining portion contains an imperfect octad repeat. Although the TrV4 gene contains only one exon, it has an intron similar in size and sequence to that of TrV1 and TrV2; in fact, the non-coding regions of all three genes show higher sequence identity than the coding regions, pointing to their common origin. Southern analysis suggests that each gene maps to a different TrIV genome segment, with homologous sequences apparently present on other segments. TrV1 and TrV4 transcription in penultimate (5th) instar hosts, parasitized shortly after the molt, was strong for both genes 1 and 2 days p.p., with transcript ...Continue Reading
References
Aug 1, 1977·In Vitro·S L Bilimoria, S S Sohi
May 24, 1991·Science·A LupasJ Stock
Dec 1, 1988·Virology·D A Theilmann, M D Summers
Jun 1, 1988·DNA·M HamelinD Pallotta
Feb 1, 1995·Preparative Biochemistry·Z Khalkhali-Ellis
Feb 1, 1995·The Journal of General Virology·M R Strand, L L Pech
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·S D Dib-HajjM D Summers
Apr 1, 1996·The Journal of General Virology·L Cui, B A Webb
Mar 22, 1996·The Journal of Biological Chemistry·I TamechikaS Hirose
Sep 1, 1996·The Journal of General Virology·M K KimD Stoltz
Nov 1, 1996·Toxicon : Official Journal of the International Society on Toxinology·T OgawaM Ohno
Jan 1, 1997·Protein Engineering·H NielsenG von Heijne
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Jun 9, 1999·Current Opinion in Chemical Biology·J Minshull, W P Stemmer
Dec 14, 1999·Molecular Microbiology·H Wu, P M Fives-Taylor
Nov 29, 2001·Biochemistry·J EinerwoldV Copié
Feb 19, 2002·Molecular and Biochemical Parasitology·Esmeralda Vargas-SerratoMary R Galinski
Mar 1, 1959·Experimental Cell Research·L M RINALDINI
Citations
Nov 16, 2004·Journal of Virology·Bertille ProvostJean-Michel Drezen
Dec 4, 2003·Annual Review of Entomology·Jeremy A Kroemer, Bruce A Webb
Jun 21, 2006·Virology Journal·L GalibertA N Volkoff
Nov 17, 2011·PloS One·Gabriel ClavijoIsabelle Darboux
Sep 9, 2010·Annual Review of Entomology·Sassan Asgari, David B Rivers
Mar 19, 2008·Archives of Insect Biochemistry and Physiology·Daniel DoucetMichel Cusson
Nov 13, 2004·Trends in Microbiology·Richard V GlatzOtto Schmidt
Jan 25, 2013·The Journal of General Virology·Abdelmadjid DjoumadMichel Cusson
Sep 15, 2006·Proteins·Michel CussonGuy H Grant
Feb 20, 2007·Virology·Kohjiro TanakaBruce A Webb
Sep 26, 2006·Advances in Virus Research·Torrence A GillBruce A Webb
Dec 21, 2004·Journal of Insect Physiology·Marianne BonvinBeatrice Lanzrein
Mar 24, 2005·The Journal of General Virology·Renée LapointeJulie A Olszewski
Dec 16, 2006·The Journal of General Virology·Daniel DoucetMichel Cusson
Oct 20, 2007·The Journal of General Virology·Don StoltzMichel Cusson
Jan 4, 2017·Journal of Insect Physiology·M Lukas SeehausenVéronique Martel
Feb 21, 2019·Molecular Immunology·Vincent ViscontiIsabelle Darboux