PMID: 12770629May 29, 2003Paper

Three related TrIV genes: comparative sequence analysis and expression in host larvae and Cf-124T cells

Journal of Insect Physiology
C BéliveauMichel Cusson

Abstract

We report on the cloning and sequencing of two Tranosema rostrale ichnovirus (TrIV) genes, and assess their relatedness to TrV1, the gene encoding the most abundant TrIV transcript in last-instar Choristoneura fumiferana larvae parasitized by T. rostrale. One of the two newly isolated genes, TrV2, features an organization similar to that of TrV1, with one intron flanked by two exons; it encodes a 102 amino acid protein showing 79% similarity to TrV1. The third gene, TrV4, encodes a larger protein (143 aa) displaying similarity to the other two only over the first approximately 50 amino acid residues of its sequence; the remaining portion contains an imperfect octad repeat. Although the TrV4 gene contains only one exon, it has an intron similar in size and sequence to that of TrV1 and TrV2; in fact, the non-coding regions of all three genes show higher sequence identity than the coding regions, pointing to their common origin. Southern analysis suggests that each gene maps to a different TrIV genome segment, with homologous sequences apparently present on other segments. TrV1 and TrV4 transcription in penultimate (5th) instar hosts, parasitized shortly after the molt, was strong for both genes 1 and 2 days p.p., with transcript ...Continue Reading

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Citations

Nov 16, 2004·Journal of Virology·Bertille ProvostJean-Michel Drezen
Dec 4, 2003·Annual Review of Entomology·Jeremy A Kroemer, Bruce A Webb
Sep 9, 2010·Annual Review of Entomology·Sassan Asgari, David B Rivers
Nov 13, 2004·Trends in Microbiology·Richard V GlatzOtto Schmidt
Feb 20, 2007·Virology·Kohjiro TanakaBruce A Webb
Sep 26, 2006·Advances in Virus Research·Torrence A GillBruce A Webb
Dec 21, 2004·Journal of Insect Physiology·Marianne BonvinBeatrice Lanzrein
Dec 16, 2006·The Journal of General Virology·Daniel DoucetMichel Cusson
Feb 21, 2019·Molecular Immunology·Vincent ViscontiIsabelle Darboux

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