Three second chromosome-linked clustered Cyp6 genes show differential constitutive and barbital-induced expression in DDT-resistant and susceptible strains of Drosophila melanogaster

Gene
S MaitraRanjan Ganguly

Abstract

The level of expression of the Cyp6a2 gene is much higher in the DDT-resistant 91-R strain than in the susceptible 91-C strain of Drosophila melanogaster (Waters et al. (1992b) Proc. Natl. Acad. Sci. USA, 89, 4855-4859). To understand the role of Cyp6a2 and related genes in insecticide resistance, we have isolated and characterized two new Cyp6 genes from the 91-R strain. The polypeptides encoded by these two genes, Cyp6a8 and Cyp6a9, show 77 and 75% amino acid sequence similarity, and 60 and 55% identity with Cyp6a2 of D. melanogaster, respectively. In the genome, Cyp6a8 and Cyp6a9 genes are closely clustered within 4 kb and map at region 51C of the second chromosome. In between them another Cyp gene is present which is more related to Cyp6a9 than to Cyp6a8. The Cyp6a8 gene which is transcriptionally highly active in 91-R, moderately active in ry506 and silent in the 91-C strain hybridizes with 2.0- and 1.8-kb RNAs. Two different-sized RNAs, 2.1 and 1.8 kb, also hybridize with the Cyp6a9 and/or Cyp6a9-related genes. While the level of 2.1-kb RNA is similar in all three strains, the level of 1.8-kb RNA is highest in the 91-R strain and barely detectable in 91-C strain. Transgenic experiments showed that a 8.3-kb BamHI fragment ...Continue Reading

References

Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·M B CohenM R Berenbaum
Dec 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·L C WatersL Y Ch'ang
Jan 1, 1995·DNA and Cell Biology·M B Cohen, R Feyereisen
Jun 1, 1994·DNA and Cell Biology·M V Frolov, V E Alatortsev

❮ Previous
Next ❯

Citations

Jul 11, 2006·Journal of Chemical Ecology·Ren Sen ZengMay R Berenbaum
Feb 11, 1999·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·J G ScottZ Wen
Feb 11, 1999·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·M AmichotJ B Bergé
Nov 24, 2001·Insect Biochemistry and Molecular Biology·S Kasai, J G Scott
Jul 12, 2002·Insect Biochemistry and Molecular Biology·Sushmita MaitraRanjan Ganguly
Jun 27, 2003·Insect Biochemistry and Molecular Biology·G Le GoffR H ffrench-Constant
Oct 8, 1999·Insect Biochemistry and Molecular Biology·J G Scott
Feb 18, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·J B BergéM Amichot
Sep 8, 2011·Genes & Development·Jyoti R MisraCarl S Thummel
Jan 25, 2005·Insect Molecular Biology·R A Festucci-BuselliB R Pittendrigh
Feb 17, 1999·Annual Review of Entomology·R Feyereisen
Apr 13, 2000·Annual Review of Entomology·J Hemingway, H Ranson
Dec 9, 2000·Annual Review of Entomology·T G Wilson
Dec 29, 1998·Emerging Infectious Diseases·W G Brogdon, J C McAllister
May 1, 2004·Proceedings of the National Academy of Sciences of the United States of America·J H F PedraBarry R Pittendrigh
Jun 29, 2010·Pesticide Biochemistry and Physiology·Maeva GiraudoRené Feyereisen
Jun 16, 2015·Insect Biochemistry and Molecular Biology·Selim TerhzazShireen-A Davies
Aug 16, 2006·Insect Molecular Biology·W SunB R Pittendrigh
Jul 10, 2004·Insect Biochemistry and Molecular Biology·Janet HemingwayHilary Ranson
Nov 14, 2006·Insect Biochemistry and Molecular Biology·Lee WilloughbyPhillip J Daborn
Nov 24, 2004·Biochemical and Biophysical Research Communications·Christian HelvigLinda L Restifo
Jul 4, 2006·Cell Metabolism·Kirst King-JonesCarl S Thummel

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.