Three-stage regulation of the amphibolic gal operon: from repressosome to GalR-free DNA

Journal of Molecular Biology
Szabolcs SemseyS Adhya

Abstract

The gal operon of Escherichia coli is negatively regulated by the Gal repressosome, a higher order nucleoprotein complex containing a DNA loop that encompasses two gal promoters. In the repressosome structure, Gal repressor (GalR) dimers are bound to the two operator sites, flanking the promoter region, thus generating a DNA loop. The DNA loop is stabilized by binding of the architectural HU protein to the apex of the loop, and negative supercoiling. The gal promoters are also regulated in opposite directions by GalR without DNA looping. The repressosome-mediated as well as looping-independent transcription regulation of the two promoters is lifted in the presence of the inducer D-galactose. We tested the effect of D-galactose on various DNA-protein and protein-protein interactions of different regulatory complexes and on transcription repression in vitro. We found that the inducer breaks up the repressosome with clear intermediates in a concentration-dependent manner. The sequential disassembly generates different stages of regulation of the gal operon. The D-galactose-dependent switch from one stage of regulation to another satisfies the amphibolic requirement of the gal operon.

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Citations

Jun 23, 2009·Proceedings of the National Academy of Sciences of the United States of America·Namiko MitaraiKim Sneppen
Oct 7, 2010·The Journal of Biological Chemistry·Péter HorváthSzabolcs Semsey
Sep 20, 2006·Nucleic Acids Research·Szabolcs SemseyKim Sneppen
Dec 26, 2006·Nucleic Acids Research·Ryan K ShultzabergerThomas D Schneider
Jun 30, 2011·MBio·Zsolt CsiszovszkiSzabolcs Semsey
Apr 7, 2007·Proceedings of the National Academy of Sciences of the United States of America·Simone PigolottiMogens H Jensen
Jul 1, 2011·Biophysical Chemistry·Sudheer TungturDorothy Beckett
Jun 30, 2009·Journal of Molecular Biology·Sandeep KrishnaSzabolcs Semsey
Mar 10, 2009·Current Opinion in Microbiology·Liskin Swint-Kruse, Kathleen S Matthews
Jul 17, 2007·Molecular Microbiology·Szabolcs SemseySankar Adhya
Jul 17, 2008·Molecular Systems Biology·Shai KaplanUri Alon
Jun 23, 2011·Molecular Microbiology·Gaëlle PorcheronCatherine Schouler
Apr 1, 2008·Molecular Cell·Shai KaplanUri Alon
Aug 1, 2007·Physical Biology·G TianaK Sneppen
Jun 10, 2016·Scientific Reports·Emil D AgerschouDaniel E Otzen
Jul 28, 2019·Protein Science : a Publication of the Protein Society·Sudheer TungturLiskin Swint-Kruse
Dec 6, 2014·Acta Crystallographica. Section D, Biological Crystallography·Do Hee KimBong Jin Lee
Sep 6, 2015·The Journal of Biological Chemistry·Sarah E BondosKathleen S Matthews

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