Three UDP-xylose transporters participate in xylan biosynthesis by conveying cytosolic UDP-xylose into the Golgi lumen in Arabidopsis

Journal of Experimental Botany
Xianhai ZhaoAi-Min Wu

Abstract

UDP-xylose (UDP-Xyl) is synthesized by UDP-glucuronic acid decarboxylases, also termed UDP-Xyl synthases (UXSs). The Arabidopsis genome encodes six UXSs, which fall into two groups based upon their subcellular location: the Golgi lumen and the cytosol. The latter group appears to play an important role in xylan biosynthesis. Cytosolic UDP-Xyl is transported into the Golgi lumen by three UDP-Xyl transporters (UXT1, 2, and 3). However, while single mutants affected in the UDP-Xyl transporter 1 (UXT1) showed a substantial reduction in cell wall xylose content, a double mutant affected in UXT2 and UXT3 had no obvious effect on cell wall xylose deposition. This prompted us to further investigate redundancy among the members of the UXT family. Multiple uxt mutants were generated, including a triple mutant, which exhibited collapsed vessels and reduced cell wall thickness in interfascicular fiber cells. Monosaccharide composition, molecular weight, nuclear magnetic resonance, and immunolabeling studies demonstrated that both xylan biosynthesis (content) and fine structure were significantly affected in the uxt triple mutant, leading to phenotypes resembling those of the irx mutants. Pollination was also impaired in the uxt triple muta...Continue Reading

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Citations

Feb 3, 2018·Journal of Integrative Plant Biology·Jun-Bo HeAi-Min Wu
Oct 13, 2018·The New Phytologist·Ruiqin ZhongZheng-Hua Ye
Apr 12, 2020·Journal of Experimental Botany·Yiping WangRuibo Hu
May 26, 2018·Journal of Experimental Botany·Georg J Seifert
Jun 13, 2019·Current Protocols in Plant Biology·Carsten RautengartenBerit Ebert
Dec 23, 2020·International Journal of Molecular Sciences·Baojia DaiAi-Min Wu
Feb 23, 2021·Journal of Integrative Plant Biology·Yuanyan ZhangJianmin Wan
May 6, 2021·Journal of Experimental Botany·Carlos M FigueroaAlberto A Iglesias
Oct 2, 2021·Frontiers in Plant Science·Ermelinda BotticellaFrancesco Sestili
Jun 16, 2021·Plant & Cell Physiology·Alban MarietteEdwin R Lampugnani

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