Three-wavelength light control of freely moving Drosophila Melanogaster for less perturbation and efficient social-behavioral studies

Biomedical Optics Express
Yen-Yin LinAnn-Shyn Chiang

Abstract

We developed a real-time automated laser-tracking system combined with continuous wave 1064-nm infrared or 473-nm blue lasers to provide punishment for studying memory in Drosophila Melanogaster. Combining optogenetic tools with laser properties, such as 473-nm and 593-nm lasers that activate light sensitive proteins in artificial transgenic flies, we can manipulate the specific neuron of an assigned fly among multiple flies to investigate neuron circuit relationships in social interactions. In restraining condition assay or optogenetic experiments, a ventral irradiated system would be more efficient due to higher ventral cuticle transmissions and neuron ganglia locations. Therefore, ventral irradiated systems cause less perturbation during behavior studies.

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Citations

Feb 26, 2016·Royal Society Open Science·Jan Bartussek, Fritz-Olaf Lehmann
May 18, 2016·Nature Methods·Dhruv GroverRalph J Greenspan
Apr 16, 2021·PLoS Genetics·Alvaro Ingles-PrietoHarald Janovjak

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