Three-way analysis of fluorescence spectra of polycyclic aromatic hydrocarbons with quenching by nitromethane

Analytical Chemistry
P D WentzellR D Guy

Abstract

The application of trilinear decomposition (TLD) to the analysis of fluorescence excitation-emission matrices of mixtures of polycyclic aromatic hydrocarbons (PAHs) is described. The variables constituting the third-order tensor are excitation wavelength, emission wavelength, and concentration of a fluorescence quencher (nitromethane). The addition of a quencher to PAH mixtures selectively reduces the fluorescence intensity of mixture components according to the Stern-Volmer equation. TLD allows the three-way matrix to be decomposed to give unique solutions for the excitation spectrum, emission spectrum, and quenching profiles for each component. The availability of spectra and calculated Stern-Volmer constants can aid in the identification of unknown components. Preprocessing of the data to correct for Rayleigh/Raman scatter and primary absorption by the quencher is necessary. Both three-component (anthracene, pyrene, 1-methylpyrene) and four-component (fluoranthene, anthracene, pyrene, 2,3-benzofluorene) synthetic mixtures are successfully resolved by TLD using quencher concentrations up to 100 mM. Results are compared using both alternating least-squares and direct trilinear decomposition algorithms. The reproducibility of e...Continue Reading

Citations

Dec 3, 2003·Journal of Chromatography. a·Valentijn G van MispelaarArian C van Asten
Jul 26, 2003·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Jian-Zhong LuRu-Qin Yu
Mar 4, 2006·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Hamid AbdollahiAbdolhossein Naseri
Apr 23, 2003·Analytical and Bioanalytical Chemistry·A Garrido FrenichJ L Martínez Vidal
Sep 3, 2021·Analytical Methods : Advancing Methods and Applications·Peter D WentzellMohsen Kompany-Zareh

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