Threonine 1342 in human topoisomerase IIalpha is phosphorylated throughout the cell cycle.

The Journal of Biological Chemistry
R IshidaA Hara

Abstract

To investigate the relationship between the modulation of topoisomerase II activity and its phosphorylation state during the cell cycle, a monoclonal antibody against C-terminal peptide (residues 1335-1350) of topoisomerase IIalpha containing a consensus sequence of casein kinase II, TDDE and its phosphorylated threonine were prepared. In an enzyme-linked immunosorbent assay, the antibody, named PT1342, recognized the immunogenic phosphopeptide but not the non-phosphorylated form of the peptide. The PT1342 antibody reacted only with a 170-kDa protein from HeLa cells and recognized anti-topoisomerase IIalpha immunoprecipitants. Furthermore, the antibody did not react with the human topoisomerase IIalpha mutated at codon 1342 from threonine to alanine, showing that PT1342 was directed against the phosphorylated threonine 1342. To examine the level of phosphorylation of threonine 1342 of topoisomerase IIalpha through the cell cycle, HeLa cells were stained simultaneously for phosphorylated topoisomerase IIalpha and DNA and analyzed by flow cytometry. Cells in the G2-M phase contained about double the PT1341-reacted topoisomerase IIalpha than did cells in G1 or S phases. The antibody stained the nuclei in interphase and mitotic chr...Continue Reading

References

Dec 1, 1992·The Journal of Cell Biology·K Shiozaki, M Yanagida
Jun 1, 1991·Current Opinion in Cell Biology·H Meisner, M P Czech
Jul 20, 1989·Journal of Molecular Biology·R A Kim, J C Wang
Jan 1, 1989·Annual Review of Biochemistry·L F Liu
Dec 17, 1989·Biochimica Et Biophysica Acta·P D'Arpa, L F Liu
May 1, 1985·The Journal of Cell Biology·W C EarnshawL F Liu
May 1, 1985·The Journal of Cell Biology·W C Earnshaw, M M Heck
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·P AckermanN Osheroff
Jan 1, 1985·Annual Review of Biochemistry·J C Wang
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·N SahyounP Cuatrecasas
Apr 20, 1986·Journal of Molecular Biology·S M GasserU K Laemmli
Dec 1, 1986·The Journal of Cell Biology·M M Heck, W C Earnshaw
Nov 1, 1988·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·W C Earnshaw
Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M SanderT Hsieh
Jan 1, 1981·Annual Review of Biochemistry·M Gellert
Feb 25, 1983·Nucleic Acids Research·M DuguetA M De Recondo
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·S DiNardoR Sternglanz
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·F M DavisP N Rao
Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·S TaageperaG J Gorbsky
Nov 1, 1994·The Biochemical Journal·P M Watt, I D Hickson
Mar 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·J RocaJ C Wang
Apr 1, 1994·Experimental Cell Research·S W SherwoodR T Schimke
May 1, 1994·Molecular and Cellular Biology·P R CaronJ C Wang

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Citations

Jun 19, 1998·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·C A Austin, K L Marsh
Mar 29, 2005·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Xianfang MengJinzhong Chen
Sep 28, 1998·Biochimica Et Biophysica Acta·R J IsaacsI D Hickson
Feb 24, 2004·Molecular Biology of the Cell·Marta AgostinhoJoão Ferreira
Dec 6, 2002·Nucleic Acids Research·Ciaran MorrisonWilliam C Earnshaw
Nov 9, 2001·Cell Structure and Function·R IshidaY Hiraoka
Mar 13, 2008·PloS One·Emma L MeczesCaroline A Austin
Jul 25, 2003·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·James W JacobbergerCharles L Goolsby
Oct 26, 2001·Japanese Journal of Cancer Research : Gann·Y MatsumotoT Fojo
Apr 10, 2002·The Journal of Biological Chemistry·Moira M MessengerDavid W Litchfield
Dec 8, 2007·The Biochemical Journal·Masato IidaHideya Komatani
Apr 23, 2008·Current Protocols in Protein Science·Tong Sun, Ralph B Arlinghaus
Jan 4, 2008·The Journal of Biological Chemistry·Hongchang LiXiaoqi Liu
Apr 11, 2021·Seminars in Cell & Developmental Biology·James R PaulsonWilliam C Earnshaw

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