Threonine aldolases: perspectives in engineering and screening the enzymes with enhanced substrate and stereo specificities

Applied Microbiology and Biotechnology
Kateryna Fesko

Abstract

Threonine aldolases have emerged as a powerful tool for asymmetric carbon-carbon bond formation. These enzymes catalyse the unnatural aldol condensation of different aldehydes and glycine to produce highly valuable β-hydroxy-α-amino acids with complete stereocontrol at the α-carbon and moderate specificity at the β-carbon. A range of microbial threonine aldolases has been recently recombinantly produced by several groups and their biochemical properties were characterized. Numerous studies have been conducted to improve the reaction protocols to enable higher conversions and investigate the substrate scope of enzymes. However, the application of threonine aldolases in organic synthesis is still limited due to often moderate yields and low diastereoselectivities obtained in the aldol reaction. This review briefly summarizes the screening techniques recently applied to discover novel threonine aldolases as well as enzyme engineering and mutagenesis studies which were accomplished to improve the catalytic activity and substrate specificity. Additionally, the results from new investigations on threonine aldolases including crystal structure determinations and structural-functional characterization are reviewed.

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Citations

Jul 12, 2016·ACS Catalysis·Nina G SchmidtWolfgang Kroutil
Feb 9, 2017·Acta Crystallographica. Section F, Structural Biology Communications·Yuki HiratoKatsushi Nishimura
Apr 13, 2018·Chembiochem : a European Journal of Chemical Biology·Karel HernándezPere Clapés
Mar 21, 2019·Frontiers in Molecular Biosciences·Jing LiangJianyong Li
May 27, 2020·Organic & Biomolecular Chemistry·Clayton M ThompsonAndrew R Buller
Jun 14, 2019·Frontiers in Bioengineering and Biotechnology·Kateryna Fesko
Jan 25, 2018·Chemical Society Reviews·Ya-Ping XueYu-Guo Zheng
Nov 11, 2019·Emerging Topics in Life Sciences·David Tew
Aug 24, 2021·Bioconjugate Chemistry·Jing ChaiMichael P Pollastri
Dec 19, 2020·ACS Chemical Biology·Prasanth KumarAndrew R Buller
Aug 26, 2021·Nature Reviews. Chemistry·Lara E Zetzsche, Alison R H Narayan
Oct 27, 2021·Chembiochem : a European Journal of Chemical Biology·Tyler J DoyonAndrew R Buller

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BETA
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BLAST

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