Threonine phosphorylation is associated with mitosis in HeLa cells

FEBS Letters
J Y ZhaoP N Rao

Abstract

Phosphorylation and dephosphorylation of proteins play an important role in the regulation of mitosis and meiosis. In our previous studies we have described mitosis-specific monoclonal antibody MPM-2 that recognizes a family of phosphopeptides in mitotic cells but not in interphase cells. These peptides are synthesized in S phase but modified by phosphorylation during G2/mitosis transition. The epitope for the MPM-2 is a phosphorylated site. In this study, we attempted to determine which amino acids are phosphorylated during the G2-mitosis (M) transition. We raised a polyclonal antibody against one of the antigens recognized by MPM-2, i.e. a protein of 55 kDa, that is present in interphase cells but modified by phosphorylation during mitosis. This antibody recognizes the p55 protein in both interphase and mitosis while it is recognized by the monoclonal antibody MPM-2 only in mitotic cells. Phosphoamino acid analysis of protein p55 from 32P-labeled S-phase and M-phase HeLa cell extracts after immunoprecipitation with anti-p55 antibodies revealed that threonine was extensively phosphorylated in p55 during G2-M but not in S phase, whereas serine was phosphorylated during both S and M phases. Tyrosine was not phosphorylated. Ident...Continue Reading

References

Dec 23, 1988·Cell·W G Dunphy, J W Newport
Oct 1, 1988·Trends in Genetics : TIG·M Lee, P Nurse
Sep 1, 1986·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R C Adlakha, P N Rao
Oct 1, 1987·Analytical Biochemistry·M DianoM Hirn
Feb 1, 1987·Molecular and Cellular Biology·M J LohkaJ L Maller
Apr 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·A B Pardee
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·T Hunter, B M Sefton
Jul 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D D VandreG G Borisy
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·F M DavisP N Rao

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Citations

Jul 4, 1998·Journal of Chromatography. a·J X YanK L Williams
Nov 1, 1993·The Journal of Cell Biology·J Kuang, C L Ashorn
Sep 1, 1996·Molecular Biology of the Cell·N Matsumoto-TaniuraJ M Westendorf
Mar 12, 2010·Molecular Biology of the Cell·Chuan Fen WuJian Kuang
Dec 14, 1990·Science·L Manuelidis
Feb 11, 2005·Analytical and Bioanalytical Chemistry·A R de BoerH Irth
Oct 20, 2006·Biochimica Et Biophysica Acta·René P ZahediAlbert Sickmann
Mar 15, 2015·Biochimica Et Biophysica Acta·David W LitchfieldLaszlo Gyenis
Jul 2, 2019·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Marvin J FritzlerMeifeng Zhang

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