Threshold conditions for synaptically evoking Ca(2+) waves in hippocampal pyramidal neurons

Journal of Neurophysiology
Suya Zhou, William N Ross

Abstract

Regenerative Ca(2+) release from inositol 1,4,5-trisphosphate (IP(3))-sensitive intracellular stores in the form of Ca(2+) waves leads to large-amplitude [Ca(2+)](i) increases in the apical dendrites of hippocampal CA1 pyramidal neurons. Release is generated following synaptic activation of group I metabotropic glutamate (mGlu) receptors. We systematically examined the conditions for evoking these waves in transverse slices from 2- to 3-wk-old rats. Using a sharpened asymmetrical bipolar tungsten stimulating electrode placed in the stratum radiatum, we varied the lateral position of the electrode, the number of stimulating pulses, the train frequency, and stimulus current. Several trends were clear. Increasing the frequency of stimulation from 20 to 100 Hz, keeping the total number of pulses constant, lowered the required stimulus current. Stimulation at frequencies below 20 Hz made it difficult to evoke release. Increasing the number of stimulation pulses, keeping the frequency constant, lowered the threshold current. A minimum of five pulses at 100 Hz was required to evoke release reliably, but several examples of success with three pulses were recorded. Theta-burst stimulation was as effective as tetanic stimulation. Placing...Continue Reading

References

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Citations

Sep 6, 2008·Proceedings of the National Academy of Sciences of the United States of America·Jordi SorianoElisha Moses
Jul 28, 2005·The European Journal of Neuroscience·Verena PawlakGeorg Köhr
Jan 18, 2006·Trends in Pharmacological Sciences·Scott BardoNigel Emptage
Nov 15, 2005·The Journal of Physiology·Clarke R Raymond, Stephen J Redman
Aug 11, 2007·The Journal of Physiology·Min Hong, William N Ross
Jul 18, 2018·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Zahid PadamseyNigel J Emptage
Mar 6, 2008·Journal of Computational Neuroscience·Bradford E Peercy
Feb 9, 2012·Nature Reviews. Neuroscience·William N Ross
Nov 15, 2003·Journal of Neurophysiology·John Connor, Lucas D Pozzo-Miller
Apr 15, 2003·The Journal of Physiology·Matthew E LarkumWilliam N Ross
Jul 18, 2018·Molecular Neurobiology·Sufyan Ashhad, Rishikesh Narayanan
Oct 3, 2012·Cold Spring Harbor Protocols·William N Ross, Satoshi Manita

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