Abstract
During Gram-negative sepsis, lipopolysaccharide (LPS) activates toll-like receptor (TLR) 4 and induces complex responses of immune system and haemostasis. In the present study we investigated whether thrombelastography is suitable to monitor the LPS-induced activation of coagulation. Whole blood samples from healthy volunteers were incubated with LPS for various incubation periods (0-5 hrs), thereafter rotation thrombelastography was performed. Incubation of whole blood (>or=3 h) with LPS markedly reduced clotting time; after 5 hrs the variable was reduced from 459+/-39 sec to 80+/-20 sec while the other thrombelastography variables (angle alpha, clot formation time, maximal clot formation) remained unaltered. EC(50) of the LPS effect on whole blood clotting time was 18 microg/ml. In isolated leukocytes, diluted in platelet poor plasma, far lower LPS-concentrations were effective: 10 ng/ml LPS reduced clotting time from 439+/-68 sec to 200+/-56 sec. Experiments with the protein synthesis inhibitor cycloheximide and active site-inhibited factor VIIa revealed that LPS exerts its effects via the synthesis of tissue factor. Addition of tissue factor to whole blood samples revealed that a concentration of 100 fmol/l can be detected ...Continue Reading
Citations
Apr 1, 2009·Critical Care : the Official Journal of the Critical Care Forum·Fritz DaudelStephan M Jakob
Oct 12, 2010·Critical Care : the Official Journal of the Critical Care Forum·Michael AdamzikMatthias Hartmann
Dec 21, 2010·Molecular Medicine·Herbert SchöchlSoheyl Bahrami
Jan 9, 2009·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Corinna Velik-SalchnerThorsten Haas
Aug 18, 2012·Acta Anaesthesiologica Scandinavica·T BrennerS Hofer
Nov 3, 2012·Perfusion·C SuckerM Hartmann
Apr 30, 2016·Perfusion·Antti LaineAlexey Schramko
Jun 17, 2010·American Journal of Clinical Pathology·Prashant Sharma, Renu Saxena
Jan 10, 2012·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Hsin-Jung TsaiChin-Chen Wu
Aug 10, 2016·PloS One·Saskia WandAlexander Koch
Mar 31, 2012·ISRN Hematology·Lutz KochPeter Ruef
Nov 12, 2010·Shock·Michael AdamzikMatthias Hartmann
Mar 16, 2013·Thrombosis and Haemostasis·J ZipperleH Schöchl
May 15, 2009·Anesthesia and Analgesia·Matthias HartmannJuergen Peters
Aug 24, 2012·Anesthesiology·Christian Friedrich WeberKai Zacharowski
Dec 20, 2012·Anesthesiology·Michael AdamzikMatthias Hartmann
Jun 18, 2017·Scientific Reports·Wolfgang HolnthonerChristoph J Schlimp
Jun 3, 2016·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Barbara AdamikAndrzej Kübler
Oct 14, 2020·Journal of Critical Care·Annalisa BoscoloPaolo Navalesi