Thrombin enhances T cell proliferative responses and cytokine production

Cellular Immunology
A NaldiniG R Klimpel

Abstract

Human alpha-thrombin, in addition to its procoagulant activity, is a mitogen for fibroblasts and endothelial cells and a chemotactic agent for monocytes. To further understand the complex physiological functions of thrombin, we investigated whether thrombin has any immunoregulatory function with regard to T cell activation. Using highly purified human alpha-thrombin and peripheral blood mononuclear cells (PBMC), we investigated whether thrombin has any effect on cytokine production and/or proliferation induced by different T cell stimuli. At physiological concentrations (1-10 micrograms/ml, 30-300 nM), thrombin significantly enhances T cell proliferation in response to mitogens, superantigens, alloantigens, and anti-CD3 stimulation. Enhanced proliferation was associated with increased IL2 and IL6 production and with an increase in the number of IL2r+ (CD25)-bearing T cells. Thrombin alone was not mitogenic nor did it induce IL2 production or increase the number of IL2r+ T cells. However, PBMC exposed to thrombin alone produced high levels of IL6. Thrombin also enhanced IL2-induced proliferation of murine and human IL2-dependent cell lines. These results suggest that thrombin may play an important role in regulating cell-mediate...Continue Reading

Citations

Nov 23, 2006·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Antonella NaldiniFabio Carraro
Jul 16, 2008·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Takeshi FujitaKen Okumura
Apr 7, 2012·Critical Care Research and Practice·Takayuki OkamotoMotomu Shimaoka
Nov 25, 2003·American Journal of Respiratory and Critical Care Medicine·Masaki TeradaNizar N Jarjour
Dec 19, 2008·Journal of the American Society of Nephrology : JASN·Akanksha GuptaBrian W Grinnell
Oct 5, 2010·Clinica Chimica Acta; International Journal of Clinical Chemistry·Luci M DusseBashir A Lwaleed
Aug 17, 2005·Journal of Thrombosis and Haemostasis : JTH·S R Coughlin
Jan 19, 2007·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·S ShrivastavaA Dorling
Oct 1, 2011·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Anthony C Bruce, Shayn M Peirce
Dec 11, 2014·IUBMB Life·Miriam Koch, Alma Zernecke
Oct 27, 2004·Peptides·Antonella NaldiniDarrell H Carney
Oct 6, 1999·Lancet·B WhiteO P Smith
Jan 5, 2010·Biochemical and Biophysical Research Communications·De-Chun ChengFeng-Min Zhang
Mar 5, 2004·Expert Opinion on Pharmacotherapy·Samir MalhotraPromila Pandhi
Mar 28, 2013·American Journal of Obstetrics and Gynecology·Alex C VidaeffHaleh Sangi-Haghpeykar
Aug 30, 2011·Thrombosis Research·S PetrosR Siegemund
Oct 23, 2003·The Journal of Biological Chemistry·Bon-Hun Koo, Doo-Sik Kim
Sep 30, 2005·Virchows Archiv : an International Journal of Pathology·Anne RullierJean Rosenbaum
Jun 24, 2008·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Offer ErezChong Jai Kim
Oct 2, 2008·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Jyh Kae NienSonia Hassan
Oct 21, 2011·Medizinische Klinik, Intensivmedizin und Notfallmedizin·S Petros, R Siegemund
Oct 23, 2002·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·T ChaiworapongsaR Romero
Aug 2, 2011·Seminars in Immunopathology·Edward M Conway
Aug 28, 2001·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·S TsunodaK Okumura
Nov 11, 2009·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Offer ErezMoshe Mazor
Oct 16, 2002·Laboratory Investigation; a Journal of Technical Methods and Pathology·Takahisa ImamuraShin Nakamura
Feb 26, 2020·International Journal of Molecular Sciences·Alexandre MansourIsabelle Gouin-Thibault
Mar 27, 2018·British Journal of Haematology·Jürgen H ProchaskaChristian Becker
Dec 31, 2002·Journal of the American Society of Nephrology : JASN·Stefan HeidenreichUlrike Nowak-Göttl
Sep 26, 2002·Thrombosis Research·Hirokazu MizuguchiTakashi Morita

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.