Abstract
Despite trauma-induced hypothermic coagulopathy being familiar in the clinical setting, empirical experimentation concerning this phenomenon is lacking. In this study, we investigated the effects of hypothermia on thrombin generation, clot formation, and global hemostatic functions in an in vitro environment using a whole blood model and thromboelastography, which can recapitulate hypothermia. Blood was collected from healthy individuals through venipuncture and treated with corn trypsin inhibitor, to block the contact pathway. Coagulation was initiated with 5pM tissue factor at temperatures 37°C, 32°C, and 27°C. Reactions were quenched over time, with soluble and insoluble components analyzed for thrombin generation, fibrinogen consumption, factor (f)XIII activation, and fibrin deposition. Global coagulation potential was evaluated through thromboelastography. Data showed that thrombin generation in samples at 37°C and 32°C had comparable rates, whereas 27°C had a much lower rate (39.2 ± 1.1 and 43 ± 2.4 nM/min vs 28.6 ± 4.4 nM/min, respectively). Fibrinogen consumption and fXIII activation were highest at 37°C, followed by 32°C and 27°C. Fibrin formation as seen through clot weights also followed this trend. Thromboelastograp...Continue Reading
References
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jun 14, 1977·Biochemistry·Y BarenholzR D Carlson
Jan 29, 1988·Biochimica Et Biophysica Acta·P J Fay
Aug 15, 1970·Nature·U K Laemmli
Jun 12, 1998·British Journal of Anaesthesia·S C KettnerM Zimpfer
Jul 31, 1999·The Journal of Biological Chemistry·K E BrummelK G Mann
Jun 8, 2002·Stroke; a Journal of Cerebral Circulation·Rüdiger GerlachVolker Seifert
Jun 19, 2002·Blood·Kathleen E BrummelKenneth G Mann
Apr 1, 1964·Archives of Biochemistry and Biophysics·L LORAND, K KONISHI
Feb 26, 2004·The Journal of Biological Chemistry·Thomas OrfeoKenneth G Mann
Mar 6, 2004·Journal of Thrombosis and Haemostasis : JTH·K E Brummel-ZiedinsK G Mann
Jun 24, 2004·The Journal of Trauma·Alisa S WolbergMaureane Hoffman
Oct 27, 2004·Anesthesia and Analgesia·Patrick WettsteinWolfgang Korte
Jun 9, 2005·Critical Care Medicine·Henry E WangSamuel A Tisherman
Oct 11, 2005·The Journal of Biological Chemistry·Thomas OrfeoKenneth G Mann
Oct 26, 2005·Journal of Thrombosis and Haemostasis : JTH·K E Brummel-ZiedinsF R Rosendaal
Nov 28, 2006·The Surgical Clinics of North America·Gregory J Jurkovich
Jun 15, 2007·The Journal of Trauma·Bijan S KheirabadiJohn B Holcomb
Sep 12, 2008·The Journal of Trauma·Wenjun Z MartiniJohn B Holcomb
Oct 14, 2008·The Journal of Trauma·Michael J ParrDavid B Hoyt
Apr 24, 2009·Annals of Surgery·Greg J BeilmanStephen M Cohn
Jul 11, 2009·The Journal of Trauma·Wenjun Zhou Martini
Aug 12, 2009·The Journal of Trauma·Myung S ParkJohn B Holcomb
Aug 12, 2009·The Journal of Trauma·Jeffry L Kashuk, Ernest E Moore
Oct 1, 2009·Surgery·Jeffry L KashukJeffrey L Johnson
Feb 24, 2012·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Jiri RuzickaAles Krouzecky
Jun 30, 2012·The Journal of Trauma and Acute Care Surgery·Matthew E KutcherMitchell Jay Cohen
Oct 3, 2012·The Journal of Trauma and Acute Care Surgery·Hasan B AlamUNKNOWN HYPOSTAT workshop participants
Citations
Oct 7, 2015·Anesthesia and Analgesia·Matthew James LawrencePhillip Adrian Evans
Nov 18, 2015·Therapeutic Hypothermia and Temperature Management·Amanda R StahlKelly N Sawyer
Sep 13, 2015·Resuscitation·Marrit JacobJesper Kjærgaard
May 1, 2016·Critical Care : the Official Journal of the Critical Care Forum·Anni Nørgaard JeppesenAnne Mette Hvas
Jun 29, 2017·ERJ Open Research·Maurizio BernasconiAlban Lovis
Oct 15, 2019·Anesthesia and Analgesia·Alexander Y MitrophanovJaques Reifman
Apr 26, 2019·Anesthesiology·Hans KirkegaardEldar Søreide
May 24, 2017·Artificial Cells, Nanomedicine, and Biotechnology·Yuchen HuangWei Xue
Feb 7, 2021·Journal of the American College of Surgeons·Julia R ColemanSaulius Butenas
Apr 18, 2021·The Journal of Thoracic and Cardiovascular Surgery·Abe DeAnda, Sean G Yates
May 12, 2021·Journal of Biomedical Materials Research. Part a·Jarel K GandhiAlan D Marmorstein