Thrombin generation by activated factor VII on platelet activated by different agonists. Extending the cell-based model of hemostasis.

Thrombosis Journal
R AltmanClaudio Gonzalez

Abstract

Platelet activation is crucial in normal hemostasis. Using a clotting system free of external tissue factor, we investigated whether activated Factor VII in combination with platelet agonists increased thrombin generation (TG) in vitro. TG was quantified by time parameters: lag time (LT) and time to peak (TTP), and by amount of TG: peak of TG (PTG) and area under thrombin formation curve after 35 minutes (AUC-->35min) in plasma from 29 healthy volunteers using the calibrated automated thrombography (CAT) technique. TG parameters were measured at basal conditions and after platelet stimulation by sodium arachidonate (AA), ADP, and collagen (Col). In addition, the effects of recombinant activated FVII (rFVIIa) alone or combined with the other platelet agonists on TG parameters were investigated. We found that LT and TTP were significantly decreased (p < 0.05) and PTG and AUC-->35min were significantly increased (p < 0.05) in platelet rich plasma activated with AA, ADP, Col, and rFVIIa compared to non-activated platelet rich plasma from normal subjects (p = 0.01). Furthermore platelet rich plasma activated by the combined effects of rFVIIa plus AA, ADP or Col had significantly reduced LT and TTP and increased AUC-->35min (but not ...Continue Reading

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Citations

Mar 3, 2010·American Journal of Respiratory and Critical Care Medicine·Sreerama ShettyRashmi S Shetty
Jan 25, 2007·Clinical Chemistry and Laboratory Medicine : CCLM·Giuseppe LippiGian Cesare Guidi
Jan 8, 2020·Journal of Clinical Medicine·Marina Panova-NoevaHugo Ten Cate

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Methods Mentioned

BETA
blood collection
Assay

Software Mentioned

thrombinoscope
Statistica
CSS

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