PMID: 6987662Feb 1, 1980Paper

Thrombin increases expression of fibronectin antigen on the platelet surface

Proceedings of the National Academy of Sciences of the United States of America
M H GinsbergE F Plow

Abstract

Fibronectins (fn) are adhesive glycoproteins which bind to collagen and to fibrin and appear to be important in cellular adhesion to other cells or surfaces. Fn-related antigen is present in human platelets, suggesting a possible role for fn in the adhesive properties of platelets. We have studied the localization of fn in resting and thrombin-stimulated platelets by immunofluorescence and quantitative binding of radiolabeled antibody. In resting fixed platelets, variable light surface staining for fn was observed. When these cells were made permeable to antibody with detergent, staining for fn was markedly enhanced and was present in a punctate distribution, suggesting intracellular localization. Stimulation with thrombin, which is associated with increased platelet adhesiveness, resulted in increased staining for fn antigen on intact platelets. These stimulated cells did not leak 51Cr nor did they stain for F-actin, thus documenting that the increased fn staining was not due to loss of plasma membrane integrity. The thrombin-induced increase in accessible platelet fn antigen was confirmed by quantitative antibody binding studies in which thrombin-stimulated platelets specifically bound 15 times as much radiolabeled F(ab')2 an...Continue Reading

References

Sep 1, 1979·The Journal of Clinical Investigation·D F Mosher, P E Schad
Aug 1, 1975·The Journal of Experimental Medicine·A Vaheri, E Ruoslahti
Apr 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·K M YamadaI Pastan
Dec 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·H B BensusanL A Culp
Sep 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J P MiletichP W Majerus
Mar 1, 1979·The Journal of Clinical Investigation·E F PlowM H Ginsberg
Jun 20, 1978·Annals of the New York Academy of Sciences·R O HynesK K Smith
Jun 1, 1978·The Journal of Experimental Medicine·E EngvallE J Miller
Oct 1, 1978·The Journal of Clinical Investigation·R L CzervionkeG L Fry
Jul 15, 1977·International Journal of Cancer. Journal International Du Cancer·E Engvall, E Ruoslahti
Nov 1, 1977·The Journal of Clinical Investigation·M H GinsbergD J McCarty
May 1, 1976·British Journal of Haematology·P N Walsh, M S Lipscomb
Jan 1, 1966·International Archives of Allergy and Applied Immunology·P J McConahey, F J Dixon
Jun 13, 1974·Biochimica Et Biophysica Acta·D R Phillips, P P Agin
Nov 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·R O Hynes

❮ Previous
Next ❯

Citations

Oct 31, 1984·Revista clínica española·M C Llena PuyG Guillén Martínez
Jul 1, 1985·Clinical & Experimental Metastasis·W A TurnerJ D Taylor
Jun 1, 1990·Clinical Rheumatology·S NishinaritaS Sawada
Jul 1, 1985·Veterinary Research Communications·A Rasedee, B F Feldman
Sep 1, 1985·Cell·J M Gardner, R O Hynes
Aug 1, 1991·Biomaterials·K Gaebel, I A Feuerstein
Jan 1, 1981·Collagen and Related Research·E RuoslahtiE G Hayman
Oct 25, 1984·The New England Journal of Medicine·J N GeorgeD R Phillips
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·D HolderbaumH Gershman
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·V M DixitW A Frazier
Mar 1, 1981·The Journal of Cell Biology·H K KleinmanG R Martin
May 1, 1982·The Journal of Cell Biology·D F MosherE A Jaffe
Nov 1, 1982·The Journal of Cell Biology·R O Hynes, K M Yamada
Feb 1, 1984·The Journal of Cell Biology·P E StenbergD F Bainton
Jan 1, 1981·The Journal of Experimental Medicine·M P BevilacquaC Bianco
Mar 1, 1983·The Journal of Clinical Investigation·M H GinsbergE F Plow
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·V M DixitW A Frazier
Jul 15, 2006·Journal of Thrombosis and Haemostasis : JTH·J Cho, D F Mosher
Feb 1, 1989·British Journal of Haematology·C J ParkerN J Bernshaw
Oct 1, 1986·British Journal of Haematology·M O SpycherM O Sypcher
Apr 22, 2016·Cellular and Molecular Life Sciences : CMLS·Yiming Wang, Heyu Ni
Feb 1, 1989·Annals of Medicine·H Holmsen
Jan 9, 1999·Perfusion·J A HydeT R Graham
Nov 1, 1981·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·H SageP Bornstein
Jan 1, 1981·Journal of Supramolecular Structure and Cellular Biochemistry·J Lahav, R O Hynes
Apr 1, 1984·The Journal of Pathology·A J D'Ardenne, J O McGee
Jan 1, 1981·Journal of Supramolecular Structure and Cellular Biochemistry·M H GinsbergJ Forsyth
Oct 15, 1984·Klinische Wochenschrift·E Klar, D L Heene
Feb 1, 1981·Journal of Oral Pathology·E Ruoslahti

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.