Thrombin-induced caspases 3 and 9 translocation to the cytoskeleton is independent of changes in cytosolic calcium in human platelets

Blood Cells, Molecules & Diseases
Nidhal B AmorAghleb Bartegi

Abstract

Apoptosis has been shown to be associated with changes in cytosolic free calcium concentration ([Ca(2+)](c)). Here we show that the agonist thrombin induces activation of caspases 9 and 3 and translocation of the caspase active forms and procaspases to the cytoskeleton in human platelets. Dimethyl-BAPTA loading did not affect thrombin-induced caspase 9 and 3 activation or translocation suggesting that these responses are independent of increases in [Ca(2+)](c). Treatment with thapsigargin plus ionomycin, to induce extensive Ca(2+) store depletion and subsequent increase in [Ca(2+)](c), stimulates caspase activation although it was unable to induce caspase translocation to the cytoskeleton. Similar results were observed in cells loaded with dimethyl-BAPTA, suggesting that activation of caspases 9 and 3 by thapsigargin plus ionomycin does not require rises in [Ca(2+)](c). These findings suggest that thrombin-induced caspase 9 and 3 activation and translocation are independent on rises in [Ca(2+)](c) but might require store depletion in human platelets.

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Citations

Apr 8, 2014·Journal of Thrombosis and Thrombolysis·Ram M ThusharaKesturu S Girish
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Feb 24, 2016·Molecular and Cellular Biochemistry·Swamy JagadishKanchugarakoppal S Rangappa

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