Thrombin-induced platelet reactions: effect of substrates and inhibitors on binding of thrombin and serotonin release

Thrombosis Research
M Iwamoto, B Blombäck

Abstract

Binding of 125I-thrombin to platelets and subsequent serotonin release were confirmed. The binding of unaltered thrombin to platelets was also measured by a new technique using a chromogenic substrate (S-2238). As compared to 125I-thrombin, this method gave similar constants for binding to the high affinity binding site, but lower for binding to the low affinity binding site. Furthermore, the results suggest that the platelets have two classes of independent binding sites. Substrates and inhibitors of thrombin inhibited thrombin induced serotonin release, suggesting that the release reaction depends on the proteolytic activity of thrombin. The serotonin release was more inhibited than the binding of thrombin, suggesting that the platelet binding site and the active site of thrombin are located in different parts of the thrombin molecule.

References

Apr 1, 1979·The Journal of Clinical Investigation·S Rittenhouse-Simmons
Oct 15, 1968·Biochimica Et Biophysica Acta·M G Davey, E F Lüscher
Jan 1, 1972·Advances in Experimental Medicine and Biology·O Tangen, H J Berman
Aug 1, 1963·The Journal of Physiology·G V BORN, M J CROSS

❮ Previous
Next ❯

Citations

Jun 1, 1993·Chemico-biological Interactions·W N Aldridge
Jan 1, 1989·The International Journal of Biochemistry·C Izquierdo, F J Burguillo
Jan 1, 1986·Toxicon : Official Journal of the International Society on Toxinology·J StürzebecherF Markwardt

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.