Abstract
We have studied the interaction between thrombin and washed, human platelets using prostacyclin, a reversible inhibitor of platelet secretion. The effect of thrombin is limited to those reactions that are not inhibited by an increased concentration of platelet cyclic adenosine 3',5'-monophosphate, because prostacyclin is a potent inducer of the latter. Prostacyclin-treated platelets were briefly (15-30 s) exposed to low concentrations of human thrombin (0.01-0.2 U/ml). After removal of the prostacyclin and thrombin, the platelets were incubated with fresh thrombin. Although they had not undergone the release reaction after the first thrombin incubation, these platelets had a diminished capacity to secrete [(3)H]serotonin when exposed to thrombin the second time. Refractoriness was concentration dependent: the higher the initial thrombin concentration, the greater the degree of inhibition of serotonin secretion on subsequent thrombin exposure. Inhibition was closely related to the ability of thrombin to induce platelet secretion and not to its esterase or fibrinogen clotting activity. Diisopropyl fluorophosphate-inactive thrombin did not induce refractoriness. Refractoriness to thrombin did not increase when the time of the init...Continue Reading
References
May 14, 1977·Lancet·K C NicolaouC M Ingerman
Mar 25, 1975·Biochemistry·F Friedman, T C Detwiler
Oct 1, 1975·The Journal of Clinical Investigation·R M LyonsP W Majerus
Mar 1, 1977·The Journal of Clinical Investigation·M MinkesP W Majerus
Mar 1, 1977·Prostaglandins·J E TatesonJ R Vane
Nov 1, 1976·Prostaglandins·R J GryglewskiJ R Vane
Nov 2, 1976·Biochemistry·B M MartinT C Detwiler
Feb 23, 1976·Biochimica Et Biophysica Acta·T K BillsM J Silver
Jan 1, 1972·The Journal of Clinical Investigation·G N BrodieP W Majerus
Nov 1, 1973·British Journal of Haematology·H J ReimersJ F Mustard
Jan 1, 1968·British Journal of Pharmacology and Chemotherapy·A Pletscher
Dec 1, 1966·The Journal of Clinical Investigation·A L WarshawN R Shulman
Mar 1, 1967·The Journal of Clinical Investigation·S Karpatkin
Dec 2, 1967·Nature·M G Davey, E F Lüscher
Citations
Oct 1, 1984·Biochemical Pharmacology·S KrishnamurthiV V Kakkar
Sep 1, 1994·Cellular Signalling·C R AlbrightsonJ M Stadel
Dec 1, 1990·British Journal of Pharmacology·D SalveminiJ Vane
Dec 1, 1987·Thrombosis Research·H J ReimersJ H Joist
Jan 1, 1986·Thrombosis Research·T ImadaY Inada
Jan 20, 1981·Biochemistry·J D IsaacsM A Shuman
Jul 16, 1991·Biochemistry·K M Lerea
Jan 1, 1987·The Journal of Surgical Research·J R KappaV P Addonizio
Aug 26, 1998·Nature·M L KahnS R Coughlin
Oct 1, 1995·Circulation Research·G Fager
Jan 1, 1986·Annals of the New York Academy of Sciences·M C BerndtP A Castaldi
Jan 1, 1983·Critical Reviews in Clinical Laboratory Sciences·M L Tiffany
Jan 1, 1986·Annals of the New York Academy of Sciences·M A Shuman
Jan 1, 1981·Annals of the New York Academy of Sciences·T C Detwiler
Mar 22, 1991·Cell·T K VuS R Coughlin
Sep 15, 1984·Thrombosis Research·M A BlajchmanM Klein