Thrombin-induced prostacyclin biosynthesis in human endothelium: role of guanine nucleotide regulatory proteins in stimulus/coupling responses

Journal of Cellular Physiology
J G GarciaK S Callahan

Abstract

The regulation of prostacyclin (PGI2) synthesis by cultured human umbilical vein endothelium (HUVEC) was investigated. HUVEC monolayer generation of PGI2 was monitored by RIA of 6-keto PGF1 alpha and dose-dependent increases observed with human alpha- and gamma-thrombins, histamine, or arachidonate. Alpha thrombin (10 nM) produced levels of 6-keto PGF1 alpha approximating responses with 1 microM gamma-thrombin, 5 microM arachidonate, or 10 microM histamine. Diisopropyl phosphorofluoridate-inactivated alpha-thrombin did not stimulate PGI2 release, demonstrating that catalytic activity was required for thrombin-stimulated PGI2 release. Sodium fluoride (NaF), at concentrations known to activate guanine nucleotide regulatory proteins (G proteins), directly stimulated HUVEC PGI2 synthesis in a dose-dependent and time-dependent manner (20 mM NaF, 4.4 +/- 0.5-fold increase at 10 min, 11.9 +/- 1.5-fold increase at 30 min). Neither alpha-thrombin nor NaF-stimulated PGI2 release was dependent upon the availability of extracellular Ca++). The hypothesis that G proteins are involved in agonist-stimulated PGI2 synthesis was further supported by studies using digitonin-permeabilized HUVEC monolayers challenged with another G protein activato...Continue Reading

References

Nov 1, 1978·The Journal of Clinical Investigation·B B WekslerE A Jaffe
May 1, 1988·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J E Allende
Mar 1, 1988·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·H HalldórssonG Thorgeirsson
Jul 1, 1988·Thrombosis Research·J G GarciaA R Johnson
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·R M BurchJ Axelrod
May 19, 1987·Biochemistry·H G DohlmanR J Lefkowitz
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·E J ForsbergH B Pollard
Jan 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S M MumbyA G Gilman
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M A ClarkJ M Stadel
Sep 30, 1986·Biochemical and Biophysical Research Communications·J MoscatP Garcia-Barreno
Jun 30, 1987·Biochemical and Biophysical Research Communications·J MoscatP García-Barreno
Jan 1, 1987·Annual Review of Biochemistry·A G Gilman
Sep 30, 1987·Biochemical and Biophysical Research Communications·S PirottonJ M Boeynaems
May 31, 1988·Biochemical and Biophysical Research Communications·M F Crouch, E G Lapetina
Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·C T Esmon, W G Owen

❮ Previous
Next ❯

Citations

Oct 6, 2000·Analytical Biochemistry·T BorbievJ G Garcia
Mar 19, 1991·Biochimica Et Biophysica Acta·E MeldrumA Carozzi
Jul 1, 1995·Trends in Cardiovascular Medicine·L F BrassJ A Hoxie
Mar 1, 1992·American Journal of Respiratory Cell and Molecular Biology·J G GarciaJ Dominguez
Jul 14, 2010·Ophthalmic Plastic and Reconstructive Surgery·Marcus M MarcetAllen M Putterman
May 31, 1990·Biochemical and Biophysical Research Communications·J F ParkinsonN U Bang
Jul 1, 1993·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·J F ParkinsonJ G Garcia
Dec 18, 2007·European Journal of Immunology·Martha TriantafilouKathy Triantafilou
Feb 8, 2018·Current Vascular Pharmacology·Andrej Fabjan, Fajko F Bajrović
Sep 1, 1996·Journal of Cardiovascular Pharmacology·J E FreemanN A Flavahan
Jun 28, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Joe G N GarciaAlexander D Verin
Apr 5, 2001·American Journal of Physiology. Cell Physiology·J G GarciaA D Verin
Sep 1, 1996·The American Journal of Physiology·H I Magazine, K D Srivastava
May 1, 1993·The American Journal of Physiology·N E TsopanoglouM E Maragoudakis
Jan 1, 1996·The Journal of Laboratory and Clinical Medicine·C M Terry, K S Callahan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.