Thrombin induces the association of cyclic ADP-ribose-synthesizing CD38 with the platelet cytoskeleton

FEBS Letters
M TortiC Balduini

Abstract

The effect of platelet stimulation on the subcellular localization of CD38, a membrane glycoprotein that catalyses the synthesis of cyclic ADP-ribose from beta-NAD+ was investigated. Treatment of human platelets with thrombin caused the association of about 40% of the total ADP-ribosyl cyclase activity with the cytoskeleton, through the translocation of the CD38 molecule from the Triton X-100-soluble to the insoluble fraction. The interaction of CD38 with the cytoskeleton was a specific and reversible process, mediated by the binding to the actin-rich filaments and was inhibited by treatment of platelets with cytochalasin D. This event was regulated by integrin alphaIIb beta3 and platelet aggregation as it was prevented by the inhibition of fibrinogen binding and was not observed in platelets from a patient affected by Glanzmann thrombasthenia. These results demonstrate that the subcellular localization of CD38 can be influenced by platelet stimulation with physiological agonists, and that membrane CD38 can interact with intracellular proteins.

References

Jan 1, 1992·International Journal of Clinical & Laboratory Research·F MalavasiS Roggero
Jan 1, 1989·Physiological Reviews·W Siess
Jul 1, 1980·The Journal of Cell Biology·D R PhillipsH H Edwards
Sep 15, 1994·Biochemical and Biophysical Research Communications·H NishinaT Katada
Aug 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·M TortiC Balduini
Jan 15, 1993·Science·A Galione
Aug 15, 1996·The Biochemical Journal·P CoorayS P Jackson

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Citations

Jul 9, 2008·The Journal of Biological Chemistry·Mirko MagnoneElena Zocchi
Sep 21, 2005·Proceedings of the National Academy of Sciences of the United States of America·Giovanna BasileElena Zocchi
Apr 1, 2021·Physiological Reviews·Alberto L HorensteinFabio Malavasi

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