Thrombin-promoted release of UDP-glucose from human astrocytoma cells.

British Journal of Pharmacology
Silvia KredaEduardo R Lazarowski

Abstract

The P2Y14 receptor is activated by UDP-sugars, most potently by UDP-glucose, but not by free nucleotides, suggesting that UDP-glucose is the cognate agonist for this receptor. However, evidence for regulated release of UDP-glucose is scarce. In the present study, the occurrence of receptor-promoted release of UDP-glucose was investigated, using 1321N1 human astrocytoma cells. UDP-glucose release and hydrolysis were measured using HPLC-based techniques. Phospholipase C activation and actin cytoskeleton reorganization were assessed by measuring inositol phosphate formation and fluorescence confocal microscopy, respectively. Thrombin and the protease-activating receptor-1 (PAR1) peptide TFLLRNPNDK (PAR1-AP) evoked the release of UDP-glucose and ATP, which was accompanied by enhanced inositol phosphate formation. Although carbachol promoted fourfold greater inositol phosphate formation than thrombin, it failed to promote nucleotide release. Thrombin-promoted nucleotide release was inhibited by BAPTA-AM, brefeldin A and cytochalasin D, and was insensitive to Pertussis toxin and PI3-kinase inhibitors. Thrombin, but not carbachol, induced actin cytoskeleton reorganization, a hallmark of Rho activation in 1321N1 cells. However, PAR-pro...Continue Reading

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Citations

May 1, 2012·Purinergic Signalling·Heike FrankePeter Illes
Apr 25, 2012·Purinergic Signalling·Eduardo R Lazarowski
Apr 25, 2012·Purinergic Signalling·Gary A WeismanLaurie Erb
Jun 26, 2013·Purinergic Signalling·Geoffrey Burnstock, Francesco Di Virgilio
May 15, 2009·The Journal of Biological Chemistry·Lucia Seminario-VidalEduardo R Lazarowski
Mar 12, 2009·The Journal of Biological Chemistry·Juliana I SesmaEduardo R Lazarowski
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Jun 8, 2012·American Journal of Physiology. Cell Physiology·Juliana I SesmaEduardo R Lazarowski
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Feb 6, 2009·American Journal of Physiology. Cell Physiology·Domenick A ProsdocimoGeorge R Dubyak
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Apr 5, 2008·Biochemical and Biophysical Research Communications·Fariborz ParandehAlbert Salehi
Aug 25, 2009·Analytical Biochemistry·Eduardo R Lazarowski
Oct 6, 2017·The Journal of Physiology·Farid G KhalafallaMark A Sussman
Apr 28, 2010·The Journal of Physiology·Silvia M KredaEduardo R Lazarowski
May 25, 2011·The Journal of Biological Chemistry·Lucia Seminario-VidalEduardo R Lazarowski
Jul 17, 2016·Purinergic Signalling·Juliana I SesmaEduardo R Lazarowski
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Jun 7, 2018·Molecular Neurobiology·Stella NyamoyaMarkus Kipp
Sep 19, 2018·International Journal of Molecular Medicine·Bing ZhaoQiao-Juan Shi

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