Thrombin regulates PDGF expression in bovine glomerular endothelial cells.

Journal of the American Society of Nephrology : JASN
G GrandalianoH E Abboud

Abstract

The proteolytic enzyme thrombin is produced during activation of the coagulation pathway. Intraglomerular fibrin deposition and thrombosis are common pathologic features of several glomerular diseases, including transplant rejection. The effect of thrombin on platelet-derived growth factor (PDGF) production and DNA synthesis in well characterized bovine glomerular endothelial cells (G/endo) was studied. DNA synthesis was measured as the amount of [3H]thymidine incorporated into acid-insoluble material. PDGF released in the supernatant was measured by Western blotting and by a radioreceptor assay. PDGF mRNA expression was analyzed by solution hybridization, using human genomic PDGF B-chain (c-sis) and A-chain cDNA probes. G/endo constitutively secrete PDGF activity in serum-free medium. Thrombin stimulates PDGF production and increases the expression of mRNA that hybridizes with labeled B-chain but not A-chain probe, whereas epidermal growth factor and transforming growth factor-alpha stimulate the expression of PDGF A-chain mRNA. In addition, thrombin stimulates DNA synthesis with a peak effect at 24 h. Unlike endothelial cells from other microvascular beds, G/endo did not respond to any of the three PDGF isoforms BB, AB, or AA...Continue Reading

Citations

May 16, 2000·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·S K ParkJ D Lee
May 23, 2000·Journal of the American Society of Nephrology : JASN·Giuseppe GrandalianoMichele Ursi
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Jun 8, 2001·Proceedings of the National Academy of Sciences of the United States of America·O I SteninaP E DiCorleto
Jun 27, 2003·Microvascular Research·Barbara CresciEnrico Magg
Dec 14, 2007·Journal of the American Society of Nephrology : JASN·Jürgen FloegeCharles E Alpers
Aug 23, 2000·The Journal of Clinical Investigation·O I SteninaP E DiCorleto
Mar 31, 2000·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·G GrandalianoF P Schena
Oct 1, 2011·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Anthony C Bruce, Shayn M Peirce

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