Thrombin-stimulated growth, clustering, and collagen lattice contraction of human gingival fibroblasts is associated with its protease activity

Journal of Periodontology
M C ChangJ H Jeng

Abstract

Thrombin is a serine protease produced following gingival tissue injury or inflammation. It regulates the functional behavior of injury-neighboring cells via the activation of specific protease-activated receptors (PAR). Thrombin's role in gingival tissue healing and inflammatory response processes is not yet well understood. We investigated the effects of thrombin on gingival fibroblast (GF) growth [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay], collagen lattice contraction, and associated morphological changes. Thrombin (>1 U/ml), but not thrombin receptor (PAR-1) agonist peptide (SFLLRN, single letter amino acid code, abbreviated as TRAP, 1 to 50 microg/ml), stimulated the growth and clustering of cultured human GF in vitro. Growth-stimulatory effects of thrombin were inhibited by D-Phe-Pro-ArgCH2Cl (PPACK), a serine protease inhibitor. By contrast, trypsin (>10 microg/ml), a PAR-2 activator, suppressed the growth of GF. Thrombin (>0.2 U/ml) and TRAP (10 to 25 microg/ml), but not trypsin, prostaglandin E2 (0.01 to 0.5 microg/ml), or bovine serum albumin (BSA) (1 to 80 microg/ml), induced the GF-populated collagen lattice contraction within 30 to 60 minutes of exposure. The thrombin-induced collag...Continue Reading

References

Mar 1, 1992·Journal of Periodontology·U M WikesjöK A Selvig
Aug 19, 1991·FEBS Letters·U B RasmussenE Van Obberghen-Schilling
Jul 1, 1991·Journal of Clinical Periodontology·T Pilot, H Miyazaki
Jul 1, 1986·Seminars in Thrombosis and Hemostasis·D H CarneyJ W Fenton
Jan 1, 1986·Annals of the New York Academy of Sciences·J W Fenton
Mar 1, 1983·Journal of Periodontology·A M Polson, M P Proye
Oct 1, 1995·Circulation Research·G Fager
Sep 1, 1995·Clinical Immunology and Immunopathology·H ShinI Maruyama
Sep 26, 1995·Proceedings of the National Academy of Sciences of the United States of America·R J SantulliP Andrade-Gordon
Feb 1, 1994·The Journal of Cell Biology·F Grinnell
Nov 1, 1993·Journal of Periodontal Research·R L MacNeil, M J Somerman
Jan 1, 1993·The Biochemical Journal·V Vouret-CraviariJ Pouysségur
Jun 15, 1993·FEMS Microbiology Letters·M A CurtisG L Howells
Feb 18, 1998·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·M C ChangJ H Jeng
May 29, 1998·Lasers in Surgery and Medicine·J TangG Godlewski
Jun 17, 1998·Proceedings of the National Academy of Sciences of the United States of America·W F XuD C Foster
Aug 26, 1998·Nature·M L KahnS R Coughlin
Nov 10, 1998·Oral Microbiology and Immunology·H K Kuramitsu

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Citations

Jun 9, 2005·Nephron. Experimental Nephrology·T D HewitsonG J Becker
Apr 14, 2015·Periodontology 2000·Matthias ChiquetDimitris Kletsas
May 16, 2009·Blood Cells, Molecules & Diseases·Kenneth R Day, Pudur Jagadeeswaran
Sep 3, 2003·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Yih-Dar NienWarren L Garner
Nov 3, 2009·Fundamental & Clinical Pharmacology·Nozomi OhuchiHajime Murakami
Jan 5, 2010·Biochemical and Biophysical Research Communications·De-Chun ChengFeng-Min Zhang
Nov 22, 2005·British Journal of Pharmacology·Jiiang-Huei JengMei-Chi Chang
Feb 3, 2005·Lasers in Surgery and Medicine·Yi-Jane ChenWan-Hong Lan
Feb 3, 2006·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·M M MurrayA Steinert
Jun 24, 2004·The Journal of Pharmacology and Experimental Therapeutics·Nobuhisa TanakaYosuke Tojyo
Aug 28, 2003·Journal of Thrombosis and Haemostasis : JTH·E B BachliJ H McVey
Apr 22, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Salunya TancharoenIkuro Maruyama

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