Thrombin stimulates activation of the cerebral 5-lipoxygenase pathway during blood-brain cell contact

Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism
M WinkingT Simmet

Abstract

The purpose of this study was to identity the trigger mechanism activating the 5-lipoxygenase pathway during blood-brain cell contact and to estimate the contribution of blood and brain cells to the cysteinyl-leukotriene (LT) biosynthesis observed under these conditions. Incubation of dissociated rat brain cells in Krebs-Henseleit solution for up to 60 min did not stimulate any detectable cysteinyl-LT biosynthesis. Incubation of recalcified rat whole blood in vitro for up to 60 min led to release of only small amounts of cysteinyl-LT into the serum samples. However, coincubation of dissociated rat brain cells with physiologically recalcified autologous whole blood triggered a time-dependent release of large amounts of immunoreactive cysteinyl-LT into the serum samples. By reverse-phase HPLC, immunoreactive cysteinyl-LT was identified as a mixture of LTC4, LTD4, and LTE4. The extent of the 5-lipoxygenase stimulation depended on the amount of autologous blood coincubated with the dissociated brain cells. Activation of the 5-lipoxygenase pathway also occurred with coincubation of dissociated rat brain cells with recalcified autologous plasma. Stimulation of cysteinyl-LT biosynthesis during blood-brain cell contact remained unaffec...Continue Reading

References

Jan 1, 1979·Thrombosis Research·C Kettner, E Shaw
Aug 1, 1992·Acta Neurologica Scandinavica·P GaetaniR Rodriguez y Baena
Apr 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·D K Banfield, R T MacGillivray
Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·L KieselB Runnebaum
Jul 1, 1991·Journal of Neurochemistry·P J Gebicke-HaerterG Hertting
Feb 1, 1990·Stroke; a Journal of Cerebral Circulation·P GaetaniG Benzi
Nov 1, 1990·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·T Simmet, B A Peskar
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·A L HultingB Samuelsson
May 1, 1987·Journal of Neurochemistry·T ShimizuY Seyama
Oct 1, 1987·Acta Neurologica Scandinavica·R RodriguezL Pacchiarini
Apr 1, 1988·Journal of the Neurological Sciences·R Rodriguez y BaenaP Paoletti
Jul 26, 1988·Brain Research·T SimmetB A Peskar
Aug 1, 1988·The American Journal of Physiology·J T Tildon, L M Roeder
Oct 1, 1988·Journal of Neurosurgery·P PaolettiR Rodriguez y Baena
Feb 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·R Bar-ShavitG D Wilner
Mar 1, 1987·Stroke; a Journal of Cerebral Circulation·M YokotaK Kokubu
Jul 1, 1985·Stroke; a Journal of Cerebral Circulation·T J DelgadoN A Svendgaard
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J A LindgrenB Samuelsson
Mar 16, 1984·European Journal of Pharmacology·A Dembinska-KiećB A Peskar
Jan 1, 1983·The Journal of Cell Biology·R Bar-ShavitG D Wilner
Jan 15, 1993·Brain Research·H EhrenreichP R Burd

❮ Previous
Next ❯

Citations

Apr 1, 2004·Clinical and Experimental Pharmacology & Physiology·Cristina C TrandafirKazuyoshi Kurahashi
Mar 28, 2008·International Journal of Environmental Health Research·Deborah A Clayton, Christopher J Griffith
Jun 11, 2014·Critical Reviews in Food Science and Nutrition·Saeed Akhtar
Jul 21, 2001·Annals of the New York Academy of Sciences·H ManevZ Zhang
Jul 6, 2000·Japanese Journal of Pharmacology·K SugayaH Manev

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.