PMID: 8943899Dec 1, 1996Paper

Thrombomodulin activity on mesothelial cells: perspectives for mesothelial cells as an alternative for endothelial cells for cell seeding on vascular grafts

British Journal of Haematology
H J VerhagenP G de Groot

Abstract

Lining the luminal surface of prosthetic small diameter bypasses with endothelial cells (EC) will lower its thrombogenicity. Unfortunately, human EC are scarce. Mesothelial cells (MC) may be a valuable alternative for EC, since they are abundantly available and have antithrombotic and fibrinolytic properties. An important anticoagulant function of EC is due to thrombomodulin (TM) on the surface. The presence of TM on omentally derived human MC is not known but would increase the chance of successful use of MC for cell seeding procedures. The expression and localization of TM on human MC was studied using monoclonal antibodies. TM activity on cultured MC, and the influence of cytokines, was measured by the generation of activated protein C (APC), and was compared to EC. TM is expressed on the surface of MC as well as intracellularly, both in vivo and in vitro. The TM-dependent generation of APC was significantly higher on cultured MC than on cultured EC (817 +/- 141 PM v 262 +/- 38 PM; P < 0.001); their reaction to cytokines was almost identical. Seeding the MC onto vascular prostheses did not change the TM activity. Thrombomodulin is present and highly active on cultured and seeded MC. This may have major implications for MC as...Continue Reading

Citations

Jul 11, 1998·European Journal of Vascular and Endovascular Surgery : the Official Journal of the European Society for Vascular Surgery·H J VerhagenB C Eikelboom
Dec 16, 2006·Endothelium : Journal of Endothelial Cell Research·Michel R HoenigJulie H Campbell
Jan 18, 2008·Journal of Biomedical Materials Research. Part a·Yang CaoJustin J Cooper-White
Feb 7, 2015·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·M VendrellC Fondevila
Dec 9, 1998·Journal of Vascular Surgery·P P Hedeman JoostenB C Eikelboom
Oct 23, 2001·Journal of Vascular Surgery·D GomesJ B Michel
Jan 20, 2018·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Amelia J HessheimerConstantino Fondevila
Feb 12, 2005·Arteriosclerosis, Thrombosis, and Vascular Biology·Michel R HoenigJulie H Campbell
Dec 11, 1999·Circulation Research·J H CampbellG R Campbell
Mar 5, 2019·Advanced Healthcare Materials·Daniel CohnRaju Ramanujan
Aug 16, 2001·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·J OhanL Drouet
Feb 17, 2017·Journal of Cardiovascular Translational Research·Wouter J GeelhoedJoris I Rotmans

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