PMID: 2496003Feb 1, 1989Paper

Thromboplastic activity of various stable cell lines

Gematologiia i transfuziologiia
O A TersenovI V Nelepchenko

Abstract

It has been established that the only source of tissue thromboplastin activity in homogenate of cells (HeLa, L-41, RAMT, Hep-2 and FL) grown in synthetic nutrient media are permolecular formations represented by fragments of cellular membranes Thromboplastic activity of nutrient media is also associated with these formations, as fragments of cellular membranes enter the media during cell cultivation. The removal of all permolecular formations from homogenate of cells, from the medium in which they are cultivated, allows determination of the topography of tissue coagulation factors.

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