PMID: 20636265Jul 20, 2010Paper

Thromboplastin immobilized on polystyrene surface exhibits kinetic characteristics close to those for the native protein and activates in vitro blood coagulation similarly to thromboplastin on fibroblasts

Biochemistry. Biokhimii︠a︡
O A FadeevaFazoil I Ataullakhanov

Abstract

A method for transmembrane protein thromboplastin (tissue factor) immobilization on polystyrene surface is described. Tissue factor is the main activating factor launching the blood coagulation process. It is a cofactor of factor VIIa, the first protease in the cascade of coagulation reactions. The proposed method preserves kinetic characteristics specific for native tissue factor on the fibroblast surface. The kinetics of binding to factor VIIa and enzymic activity of the formed complex follow Michaelis-Menten kinetics, which is also characteristic of native complex. A small difference is that dissociation constant for tissue factor immobilized on polystyrene surface exceeds 2.7-fold that for native factor. The proposed technique of immobilization provides for protein density on the activating surface corresponding to the tissue factor density on the fibroblast surface. The immobilized tissue factor can be used to activate blood coagulation in methods simulating spatial dynamics of in vitro clot growth. Investigation in this direction will make it possible to register both hypo- and hypercoagulation states of the system. This approach is advantageous over traditional methods of estimation of the coagulation system conditions, ...Continue Reading

References

Feb 14, 2002·Biochemistry. Biokhimii︠a︡·S Butenas, K G Mann
Sep 3, 2002·Biochimica Et Biophysica Acta·Mikhail V OvanesovFazly I Ataullakhanov
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Citations

Nov 30, 2011·PloS One·Thomas OrfeoKenneth G Mann
Aug 19, 2015·Thrombosis Research·Mikhail A PanteleevFazoil I Ataullakhanov
Dec 29, 2013·Thrombosis Research·Natalia M DashkevichClaude Negrier
Jan 31, 2015·Thrombosis Journal·Elena N Lipets, Fazoil I Ataullakhanov
Dec 17, 2015·PloS One·Vladimir N KolyadkoMikhail A Panteleev
Apr 14, 2017·Scientific Reports·Elena I SinauridzeFazoil I Ataullakhanov
Jul 8, 2017·PloS One·Ansar S ZhalyalovAlexey M Shibeko
Dec 26, 2018·Arteriosclerosis, Thrombosis, and Vascular Biology·Dmitry Y NechipurenkoMikhail A Panteleev
Jun 13, 2012·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Natalia P SoshitovaFazoil I Ataullakhanov
Nov 20, 2013·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Irina V GribkovaElena I Sinauridze
Apr 7, 2020·Current Pharmaceutical Design·Mikhail A PanteleevAlexey I Lobanov
Jun 28, 2015·Briefings in Bioinformatics·Alexey M Shibeko, Mikhail A Panteleev
Sep 14, 2020·Thrombosis and Haemostasis·Debora Bertaggia CalderaraLorenzo Alberio
Sep 25, 2014·Biomedit︠s︡inskai︠a︡ khimii︠a︡·I A ShcherbinaF I Ataullakhanov

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