PMID: 9433377Jan 20, 1998Paper

Thrombopoietin and erythropoietin activate inside-out signaling of integrin and enhance adhesion to immobilized fibronectin in human growth-factor-dependent hematopoietic cells

Annals of Hematology
A GotohH E Broxmeyer

Abstract

Erythropoietin (EPO) and thrombopoietin (c-MPL ligand; TPO) are structurally similar cytokines and support respectively, the proliferation and differentiation for erythroid and megakaryocytic lineages, as well as more primitive progenitors. We studied the effect of these cytokines on the induction of adhesion of human growth-factor-dependent hematopoietic cells to immobilized fibronectin, which is a main component of the extracellular matrix in the bone marrow. MO7ER cells that are genetically engineered to express human EPO receptor and MO7e cells that express endogenous c-MPL were used. Stimulation with either TPO or EPO induced rapid increases in adhesion of M07ER cells to fibronectin without apparent change of expression of integrins. Experiments with inhibitory monoclonal antibodies (mAbs) demonstrated that CD41, which has been reported to be involved in TPO-induced adhesion of megakaryocytic cells, is not responsible for this enhanced adhesion. Anti-beta 1 integrin mAb inhibited adhesion completely, while inhibition by anti-alpha 4 integrin mAb and anti-alpha 5 integrin mAb was partial. Combination of anti-alpha 4 mAb plus anti-alpha 5 mAb completely abolished adhesion, as did anti-beta 1 mAb, suggesting that the adhesion...Continue Reading

Citations

Jun 18, 1999·Annals of the New York Academy of Sciences·C R MantelH E Broxmeyer
Jul 8, 1999·Proceedings of the National Academy of Sciences of the United States of America·M YagiS Bartelmez
Jun 20, 2007·Experimental Hematology·Ian S Hitchcock, Kenneth Kaushansky
Jun 8, 2014·European Journal of Pharmacology·Zhan LingMo Wei
Aug 26, 2003·Molecular Cancer·Martin P KracklauerGuido M Sclabas
Sep 24, 2004·Physiological Reviews·Michael C Brown, Christopher E Turner
Nov 6, 2001·Immunopharmacology and Immunotoxicology·H M KimN H An

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.