PMID: 11922908Mar 30, 2002Paper

Thrombostatin, a bradykinin metabolite, reduces platelet activation in a model of arterial wall injury

Cardiovascular Research
Alejandro R PrietoGeorge S Abela

Abstract

Thrombin activates platelets and contributes to the occlusion of arteries following thrombolytic therapy or angioplasty. Thrombostatin (RPPGF), the angiotensin converting enzyme degradation product of bradykinin, inhibits alpha-thrombin induced platelet activation. We hypothesized that thrombostatin prevents platelet aggregation and adhesion after balloon angioplasty (BA). Platelet-rich plasma (PRP) was obtained from 22 Beagle dogs before sacrifice and 10% of the PRP was labeled with 111In. Carotid arteries were then removed from each dog and mounted in a dual perfusion chamber and intimal injury was performed with BA. 111In-PRP with or without thrombostatin or aspirin alone was perfused through the arteries for 60 min. During perfusion, platelet volume was measured using a Coulter counter and a laser-light scattering technique. Platelet adhesion to arteries was measured by radioactivity count. Arterial injury alone compared to non-injury increased platelet volume in the circuit by 1.4 times (x) (P<0.05) using a Coulter counter or 1.8x (P<0.05) using laser-light scattering and increased platelet adhesion by 2.3x (P<0.01). When compared to BA injury alone, the addition of thrombostatin reduced platelet volume by 1.8x (P<0.03) as...Continue Reading

Citations

Dec 20, 2002·International Immunopharmacology·Zia Shariat-MadarAlvin H Schmaier
Jan 27, 2005·Photomedicine and Laser Surgery
Apr 4, 2006·Drug Discovery Today·Dominic J AutelitanoMathew Vadas
May 2, 2003·Expert Opinion on Investigational Drugs·Johannes Ruef, Hugo A Katus
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