Thromboxane mediates neutrophil superoxide production in pregnancy.

American Journal of Obstetrics and Gynecology
J E VaughanGeorge D Ford

Abstract

We recently reported that activation of neutrophils obtained from pregnant women resulted in production of tumor necrosis factor-alpha by a thromboxane- and cyclooxygenase-2- dependent mechanism. Activated neutrophils also generate reactive oxygen species such as the superoxide anion, which can lead to oxidative damage of biomolecules. In this study, we tested the possibility that thromboxane plays a role in neutrophil superoxide generation in pregnancy via cyclooxygenase-2 by inhibiting key enzymes in the pathway leading to its synthesis. Neutrophils were isolated from normal pregnant women and incubated for 2 hours in phosphate-buffered saline with glucose alone or with treatments. Experiment 1 treatments were: (1) indomethacin at a dose sufficient to inhibit phospholipase A2 (100 microM); (2) aspirin (100 microM), a cyclooxygenase-1 and cyclooxygenase-2 inhibitor; (3) NS-398 (10 microM), a specific cyclooxygenase-2 inhibitor; (4) nordihydroguaiaretic acid (10 microM), a lipoxygenase inhibitor; and (5) pinane thromboxane, a thromboxane synthase inhibitor. Experiment 2 treatments were arachidonic acid (50 microM and 100 microM) and arachidonic acid (100 microM) alone or in combination with phorbol myristic acid plus varying do...Continue Reading

References

Aug 1, 1989·British Journal of Obstetrics and Gynaecology·I A GreerA A Calder
Dec 1, 1993·American Journal of Obstetrics and Gynecology·S W Walsh, Y Wang
Jan 1, 1997·Clinical Science·A BardenC A Michael
Jul 6, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S W WalshL J Roberts
Oct 20, 2001·American Journal of Obstetrics and Gynecology·M T GervasiR Romero
Jun 1, 2005·Free Radical Biology & Medicine·Toryn M PoolmanMargaret M Manson
Sep 10, 2005·American Journal of Obstetrics and Gynecology·John E Vaughan, Scott W Walsh

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Citations

Sep 29, 2011·Hypertension·Nikita MishraScott W Walsh
Mar 12, 2010·Reproductive Sciences·Teddi BachawatyScott W Walsh
Aug 23, 2008·Nutrition & Metabolism·Yukiko K NakamuraStanley T Omaye
Mar 10, 2016·Pregnancy Hypertension·Salih SerinHakan Kıran
Nov 21, 2007·Trends in Endocrinology and Metabolism : TEM·Scott W Walsh
Nov 17, 2009·Journal of Thrombosis and Haemostasis : JTH·F PassamontiM Cazzola
Nov 13, 2008·Hypertension in Pregnancy : Official Journal of the International Society for the Study of Hypertension in Pregnancy·Kristen A Cadden, Scott W Walsh
Feb 26, 2019·BioMed Research International·Svetlana Verenich, Phillip M Gerk
Jul 3, 2021·International Journal of Molecular Sciences·Scott W Walsh, Jerome F Strauss

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