Thy-1 modulation and cell proliferation at early steps of intrathymic bone marrow cell differentiation

Molecular Immunology
S EzineC Penit

Abstract

Intrathymic (IT) transfer of bone marrow (BM) precursor cells in sublethally irradiated hosts has been widely used to study T cell differentiation and maturation. In this report we have used double congenic mice Ly 5.1 Thy 1.1 (host) and Ly 5.2 Thy 1.2 (donor) and detected cycling Ly 5.2+ BM cells by in vivo bromodeoxyuridine incorporation, before induction of the Thy 1.2 antigen. Until Day 9 post-transfer, some donor type cells express a high level of Thy 1.2 together with macrophage and granulocyte markers. A few days later, a Thy 1.2low population transiently B220+ was detected. Thereafter, donor type cells expressed an intermediate Thy 1.2 brightness; this population then persisted and surpassed the other subsets. Our findings permitted to establish a relationship between cell cycle and Thy 1 fluorescence intensity according to the sequence: Thy 1low resting, Thy 1low cycling, Thy 1high cycling, Thy 1high resting. Moreover, we have shown that cells from the myeloïd and B lineages can, in vivo, transiently express the Thy 1 antigen, develop and differentiate within the thymus microenvironment.

References

Sep 1, 1993·Immunology Today·R L BoydP Hugo

Citations

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Related Concepts

T-Lymphocyte
Neoplasm of Uncertain or Unknown Behavior of Thymus
Bone Marrow
Disease of Thymus Gland
Biologic Development
PTPRC wt Allele
Genetic Markers
Thymic Tissue
Cell Proliferation
Lymphocyte Activation

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