PMID: 8046341Aug 1, 1994Paper

Thymic and extrathymic origins of gut intraepithelial lymphocyte populations in mice

The Journal of Experimental Medicine
Benedita RochaD Guy-Grand

Abstract

We have investigated the origin of intraepithelial lymphocytes (IEL) populations in the murine gut, using reconstitution experiments in which the presence of thymus-derived cells of host or donor origin is rigorously controlled: RAG-/- mutant mice which have no T cells, were injected either with the bone marrow (BM) cells of nude mice or with selected peripheral lymph node (LN) T cells of euthymic mice. In thymectomized RAG-/- mice, injection of BM cells from nude mice led, after 2 mo, to the development of a peripheral B cell compartment and to the appearance, in the gut, of IEL bearing homodimeric CD8 alpha chains and either gamma/delta or alpha/beta TCR. In RAG-/- mice with a thymus, a similar injection led to complete lymphoid reconstitution, with the additional appearance in the gut of CD4+, CD8 alpha/beta+ or CD4+CD8 alpha/alpha+ IEL, all bearing alpha/beta TCR. In contrast, injection of LN T cells into these mice reconstituted a gut IEL population made of CD4+, CD8 alpha/beta+, or CD4+ CD8 alpha/alpha+ cells, all bearing alpha/beta TCR; CD8 alpha/alpha+ TCR-gamma/delta+ or alpha/beta+ IEL were not observed. These results demonstrate that the thymus and/or thymic-derived peripheral T cells are absolutely required for the ...Continue Reading

References

Nov 1, 1992·Immunology Today·B RochaD Guy-Grand
Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·B RochaD Guy-Grand
Mar 8, 1991·Science·B Rocha, H von Boehmer
Jan 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·A BandeiraS Tonegawa
May 1, 1986·The Journal of Clinical Investigation·D Guy-Grand, P Vassalli
Jun 1, 1974·European Journal of Immunology·D Guy-GrandP Vassalli

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Citations

Dec 18, 2001·European Journal of Immunology·J Woodward, E Jenkinson
Sep 24, 1999·Immunologic Research·I N Crispe
Sep 14, 2013·Seminars in Immunopathology·Jörg Stange, Marc Veldhoen
Dec 16, 2000·Springer Seminars in Immunopathology·C MillerA C Hayday
Nov 19, 2008·Cell and Tissue Research·Masaki OgataTsunetoshi Itoh
Feb 13, 2008·Cell Biology and Toxicology·Jose A Aquino EsperanzaNora Cristina Brandan
Jan 1, 1995·Immunology Today·L Lefrançois, L Puddington
Apr 1, 1995·Current Opinion in Immunology·B RochaP Vassalli
May 14, 2005·Fish & Shellfish Immunology·J H W M RomboutG Scapigliati
Aug 2, 2003·Gastroenterology·Francisco LeónGarbiñe Roy
Apr 17, 2003·The Journal of Surgical Research·Hua YangDaniel H Teitelbaum
May 24, 2003·Experimental Hematology·Marie-Eve BlaisClaude Perreault
May 20, 2003·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·E QuinnR Ward
Oct 31, 2001·Nature Immunology·A HaydayJ Shires
Apr 2, 2004·Nature Reviews. Immunology·Hilde Cheroutre, Loui Madakamutil
Jun 15, 1995·The New England Journal of Medicine·G Cunnane, C O'Farrelly
Jun 20, 1995·Proceedings of the National Academy of Sciences of the United States of America·H KomanoS Itohara
Jan 17, 1995·Proceedings of the National Academy of Sciences of the United States of America·J P DiSantoK Rajewsky
Apr 1, 1997·Proceedings of the National Academy of Sciences of the United States of America·C Y KuanP Rakic
Oct 1, 1994·The Journal of Experimental Medicine·P Poussier, M Julius
Mar 8, 2000·The Journal of Experimental Medicine·M K KennedyJ J Peschon
Feb 21, 2002·The Journal of Experimental Medicine·Florence LambolezBenedita Rocha
Jun 5, 2002·The Journal of Experimental Medicine·Philippe PoussierMichael Julius

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