PMID: 273245Feb 1, 1978Paper

Thymic hormone-like restoration by human prealbumin of azathioprine sensitivity of spleen cells from thymectomized mice

Proceedings of the National Academy of Sciences of the United States of America
P BurtonA White

Abstract

Gel filtration of fresh human serum revealed that over 95% of the thymic-hormone-like activity was present in the fraction representing the total albumin and prealbumin. Further studies demonstrated that the activity resided in the prealbumin fraction; albumin was inactive. Prealbumin was isolated from Cohn fraction IV-1 of pooled human plasma by extraction with aqueous buffer, fractionation with (NH4)2SO4, gel filtration on Sephadex G-150, and preparative disc gel electrophoresis. The final product was homogeneous by analytical gel electrophoresis was 40,000 times more active than the starting material in an azathioprine-sensitive rosette assay. Physical and chemical characterization studies showed that the isolated product was identical to authentic human plasma prealbumin. Isolation of the prealbumin fraction from sera of adult thymectomized mice revealed that the rosette activity was substantially lower than that isolated from sera of normal mice, suggesting a thymic dependence of the prealbumin activity. In vitro and in vivo bioassays of the fraction obtained prior to the final step of the purification procedure support the conclusion that prealbumin exhibits thymic hormone-like activity.

References

Nov 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·J F BachA White
Jul 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·A L GoldsteinA White
Sep 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·A L GoldsteinA White
Dec 1, 1968·Analytical Biochemistry·N Catsimpoolas

Citations

Oct 23, 1980·Clinica Chimica Acta; International Journal of Clinical Chemistry·P J LisiM Schiffman
Jan 1, 1985·International Journal of Immunopharmacology·P BurtonA White
Aug 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R ReichhartH Jörnvall
Jan 30, 2003·Clinical Chemistry and Laboratory Medicine : CCLM·Joana Almeida Palha
Jan 1, 1984·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·G BussolatiA Sapino
Jan 1, 1985·Critical Reviews in Oncology/hematology·R S Schulof
Jan 1, 1979·Annals of the New York Academy of Sciences·J F BachJ M Pleau
Jan 1, 1979·Annals of the New York Academy of Sciences·A White, P Burton
Jul 14, 1987·Biochemistry·T PetterssonH Jörnvall
Feb 1, 1980·European Journal of Immunology·M DardenneJ F Bach
Jan 1, 1979·Journal of Immunopharmacology·J F Bach
Jan 4, 1988·FEBS Letters·J Gómez-Márquez, F Segade

Related Concepts

Azathioprine Sulfate
Mice, Inbred C57BL
Prealbumin
Immunocytoadherence
Plasma Albumin
Spleen
Thymectomy
Thymosin Fs
Thymus Hormones

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