Thymidine block does not synchronize L1210 mouse leukaemic cells: implications for cell cycle control, cell cycle analysis and whole-culture synchronization.

Cell Proliferation
Stephen CooperS Ravi

Abstract

It has been predicted that whole-culture methods of synchronization cannot synchronize cells. We have tested whether thymidine block, one type of whole-culture synchronization, can synchronize L1210 cells. We demonstrate experimentally that the thymidine block method cannot produce a synchronized culture. Although thymidine-treated cells are arrested primarily with an S-phase amount of DNA, there is no narrowing of the cell size distribution and there is no synchronized division pattern following release from the thymidine block. In contrast to a whole-culture synchronization method, cells produced by a selective (i.e. non-whole-culture) method not only have a specific DNA content, but also have a narrow size distribution and divide synchronously. Generalizing the results to other cell lines, we suggest that these conclusions call into question experimental measurements of gene expression during the division cycle based on thymidine inhibition synchronization.

References

Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·A L KungR T Schimke
Jan 1, 1993·Molecular and Cellular Biology·J W LudlowJ A DeCarprio
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·P Jansen-DürrM Eilers
Mar 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Kerby Shedden, Stephen Cooper
May 22, 2002·BioTechniques·Maureen ThorntonCharles E Helmstetter
Jun 12, 2002·Molecular Biology of the Cell·Michael L WhitfieldDavid Botstein
Aug 15, 2002·Cell Biology International·Stephen Cooper
Mar 13, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Stephen Cooper
Apr 16, 2003·Cell Cycle·Charles E HelmstetterK Leigh Eward
Jul 16, 2003·Cellular and Molecular Life Sciences : CMLS·S Cooper
Oct 28, 2003·Cell & Chromosome·Stephen Cooper, Kerby Shedden
May 26, 2004·Trends in Biotechnology·Stephen Cooper
May 26, 2004·Trends in Biotechnology·Paul T Spellman, Gavin Sherlock
May 26, 2004·Trends in Biotechnology·Paul T Spellman, Gavin Sherlock
Jul 16, 2004·Cell Cycle·K Leigh EwardCharles E Helmstetter
Mar 21, 2006·Experimental Eye Research·Sara J LiliensiekChristopher J Murphy
Sep 26, 2006·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Stephen Cooper

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Citations

Oct 17, 2008·Proceedings of the National Academy of Sciences of the United States of America·Iain G DugginStephen D Bell
Sep 19, 2008·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Stephen Cooper
Mar 20, 2014·Applied Microbiology and Biotechnology·Uwe JandtAn-Ping Zeng
Jun 26, 2021·Advanced Healthcare Materials·Christina Cortez-JugoFrank Caruso

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