Thymine and guanine base specificity of human myeloma proteins with anti-DNA activity

The Journal of Clinical Investigation
M Zouali, B D Stollar

Abstract

To further our understanding of the molecular basis of DNA-autoantibody interactions, we have characterized the specificities of three IgG human myeloma proteins that bind DNA. We measured their binding to synthetic single- and double-stranded homopolynucleotides, random and alternating copolymers, oligonucleotides, and nucleotides or nucleosides conjugated to non-nucleic acid carriers. All three antibodies bound single-stranded nucleic acids, including both polyribonucleotides and polydeoxyribonucleotides. They varied in relative affinities for polynucleotides of varying base composition. Polymers containing the purines guanine or hypoxanthine and/or the pyrimidine thymine were most reactive with all three proteins. A myeloma protein that reacted with poly(G), poly(I), or poly(dT) also bound to the corresponding nucleosides or nucleotides conjugated to bovine serum albumin. None of the antibodies reacted with base-paired double-helical polynucleotides (double-stranded RNA, RNA-DNA hybrid or double-stranded DNA). The results indicate that base specificity is prominent in their reactions and that the accessible epitopes in single-stranded polynucleotides become masked upon base pairing in double-stranded helices. These findings ...Continue Reading

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Citations

Mar 1, 1990·Clinical Rheumatology·B D Stollar
Jan 1, 1989·Immunologic Research·M Aldo-BensonY Borel
Mar 15, 1989·Clinica Chimica Acta; International Journal of Clinical Chemistry·C K Osterland
May 1, 1993·Free Radical Biology & Medicine·K FrenkelE Taioli
Jan 1, 1989·International Reviews of Immunology·B D Stollar
Oct 1, 1988·Immunological Reviews·M ZoualiR S Schwartz
Oct 3, 2012·International Immunopharmacology·Lynnae M SmithDavid A Loeffler
May 30, 1989·Biochemical and Biophysical Research Communications·T W Munns, S K Freeman
Jun 1, 1988·European Journal of Immunology·M ZoualiB D Stollar
Jan 1, 1988·Critical Reviews in Clinical Laboratory Sciences·M PradellaF Rigolin

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