Thymineless death in Escherichia coli: deoxyribonucleic acid replication and the immune state.

Journal of Bacteriology
D J Cummings, A R Kusy

Abstract

Thymineless death (TLD) and nalidixic acid (NA) inactivation were studied in multiple auxotrophic strains of Escherichia coli B and B/r. As expected, it was found that both E. coli B and B/r exhibited an "immune state," i.e., a fraction of the population survived inactivation to both TLD and NA. With glucose as a carbon source in minimal medium, 0.1 to 0.3% of strain B and 0.2 to 0.5% of strain B/r survived inactivation; with acetate as the carbon source, the surviving fractions were increased to 1 to 2% and 5 to 7%, respectively. These immune fractions could be increased in magnitude by preincubation in minimal media containing thymine. Systematic analysis of the particular supplements necessary for the immune state indicated that the absence of the required amino acids was essential for the maximal expression of immunity. However, immunity was not abolished in acetate medium even in the presence of the required supplements. Further studies on the replication of deoxyribonucleic acid (DNA) during preincubation indicated that the degree of immunity did not necessarily correlate with the completion of a round of DNA replication. This finding was supported by examining the immune state in synchronous populations. In both glucose ...Continue Reading

Citations

Jan 1, 1973·Zeitschrift für allgemeine Mikrobiologie·G Strohbach
Jan 19, 1999·Annual Review of Microbiology·S I AhmadA Eisenstark
Oct 1, 1970·Journal of Bacteriology·R Seale, D J Weber
Aug 1, 1973·Journal of Bacteriology·C M Berg, J M O'Neill
Oct 1, 1973·Antimicrobial Agents and Chemotherapy·G J BourguignonR Sternglanz

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