Thymopoietin radioreceptor assay utilizing lectin-purified glycoprotein from a biologically responsive T cell line

Archives of Biochemistry and Biophysics
T K AudhyaG Goldstein

Abstract

Radiolabeled thymopoietin that was biologically active and of high specific activity was prepared by a novel technique involving protection of free amino groups, selective excision of the protected N-terminal prolyl group with post-proline cleaving enzyme, reaction of the newly exposed alpha-amino group with a highly radioiodinated compound, and deprotection and purification of the polypeptide. Binding of this radiolabeled thymopoietin was not demonstrable by conventional techniques with cells, cell membranes, or solubilized cell membranes, apparently due to the presence of active proteases in these preparations. A glycoprotein with thymopoietin binding properties was prepared by lectin purification from the detergent-solubilized membranes of CEM cells, a human T cell line that responds to thymopoietin in vitro with increases in intracellular cyclic GMP. Presumably this procedure separated the thymopoietin binding protein from membrane proteases, thus permitting the development of a radioreceptor assay. Evidence is presented that the thymopoietin binding protein represents a thymopoietin receptor that is probably related to the mediation of immunoregulatory actions of thymopoietin on a subset of peripheral T cells.

References

Jun 25, 1998·Immunologic Research·V K SinghS S Agarwal
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May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·K VenkatasubramanianG Goldstein
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Citations

Jan 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·G GoldsteinE A Boyse
Jan 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J R GavinD N Buell
Apr 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·R S Basch, G Goldstein
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·T K AudhyaG Goldstein
Oct 1, 1982·The Journal of Experimental Medicine·G E RangesM P Schield
Dec 28, 1971·Biochimica Et Biophysica Acta·J I Thorell, B G Johansson

Related Concepts

TMPO gene
T-Lymphocyte
Tissue Membrane
Cyclic GMP Measurement (Lab Test)
Isolectins
Glycoproteins
Protoplasm
Peptide Hydrolases
Endopeptidases
Proteolytic Enzyme

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