Thymoquinone strongly inhibits fMLF-induced neutrophil functions and exhibits anti-inflammatory properties in vivo

Biochemical Pharmacology
Kaouthar BoudiafPham My-Chan Dang

Abstract

Polymorphonuclear neutrophils are key players in host defense against pathogens through the robust production of superoxide anion by the NADPH oxidase and the release of antibacterial proteins from granules. However, inappropriate release of these agents in the extracellular environment induces severe tissue injury, thereby contributing to the physiopathology of acute and chronic inflammatory disorders. Many studies have been carried out to identify molecules capable of inhibiting phagocyte functions, in particular superoxide anion production, for therapeutic purposes. In the present study, we show that thymoquinone (TQ), the major component of the volatile oil from Nigella sativa (black cumin) seeds strongly inhibits fMLF-induced superoxide production and granules exocytosis in neutrophils. The inhibition of superoxide anion was not due to a scavenger effect, as TQ did not inhibit superoxide anion produced by the xanthine/xanthine oxidase system. Interestingly, TQ impaired the phosphorylation on Ser-304 and Ser-328 of p47(PHOX), a cytosolic subunit of the NADPH oxidase. TQ also attenuated specific and azurophilic granule exocytosis in fMLF-stimulated neutrophils as evidenced by decreased cell surface expression of gp91(PHOX) a...Continue Reading

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Citations

May 30, 2017·Molecular and Cellular Biochemistry·Ravikanth VelagapudiOlumayokun A Olajide
Mar 18, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Fernanda PetryLiz G Müller
Mar 21, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Zeynab KohandelSaeed Samarghandian
Mar 23, 2021·Food Science & Nutrition·Masood Sadiq ButtZaffar Mehmood

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