Thymosin Beta-4 Suppresses Osteoclastic Differentiation and Inflammatory Responses in Human Periodontal Ligament Cells

PloS One
Sang-Im LeeEun-Cheol Kim

Abstract

Recent reports suggest that thymosin beta-4 (Tβ4) is a key regulator for wound healing and anti-inflammation. However, the role of Tβ4 in osteoclast differentiation remains unclear. The purpose of this study was to evaluate Tβ4 expression in H2O2-stimulated human periodontal ligament cells (PDLCs), the effects of Tβ4 activation on inflammatory response in PDLCs and osteoclastic differentiation in mouse bone marrow-derived macrophages (BMMs), and identify the underlying mechanism. Reverse transcription-polymerase chain reactions and Western blot analyses were used to measure mRNA and protein levels, respectively. Osteoclastic differentiation was assessed in mouse bone marrow-derived macrophages (BMMs) using conditioned medium (CM) from H2O2-treated PDLCs. Tβ4 was down-regulated in H2O2-exposed PDLCs in dose- and time-dependent manners. Tβ4 activation with a Tβ4 peptide attenuated the H2O2-induced production of NO and PGE2 and up-regulated iNOS, COX-2, and osteoclastogenic cytokines (TNF-α, IL-1β, IL-6, IL-8, and IL-17) as well as reversed the effect on RANKL and OPG in PDLCs. Tβ4 peptide inhibited the effects of H2O2 on the activation of ERK and JNK MAPK, and NF-κB in PDLCs. Furthermore, Tβ4 peptide inhibited osteoclast differen...Continue Reading

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Citations

Feb 15, 2019·Journal of Clinical Periodontology·Juliane SchulzDominik M Schulte
May 12, 2018·Yonsei Medical Journal·Joo Heon YoonSeung Joon Baek
Aug 16, 2017·Biomedical Reports·Kyoung Soo Kim, Hyung-In Yang
May 18, 2017·International Journal of Molecular Sciences·Yanli LiuYongjin Chen
Jun 12, 2021·Journal of Gastroenterology and Hepatology·ZhenKun YanLiqiang Wang

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Methods Mentioned

BETA
ELISA
PCR
electrophoresis
nuclear translocation

Software Mentioned

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