Thymosin beta4 improves functional neurological outcome in a rat model of embolic stroke.

Neuroscience
Daniel C MorrisZ Zhang

Abstract

Thymosin beta4 (Tbeta4) is a developmentally expressed 43-amino acid peptide that inhibits organization of the actin-cytoskeleton by sequestration of G-actin monomers. Tbeta4 improves cardiac function after myocardial infarction in adult mice and promotes healing properties in both dermal and corneal wounds. We tested the hypothesis that Tbeta4 improves functional neurological outcome in a rat model of embolic stroke. Male Wistar rats (n=18) were subjected to embolic middle cerebral artery occlusion (MCAo). Tbeta4 (6 mg/kg, IP) was administered 24 h after MCAo and then every 3 days for four additional doses (n=9). Rats treated with saline were used as a control (n=9). The adhesive-removal test (ART) and modified Neurological Severity Score (mNSS) were performed to measure functional outcome. Rats were sacrificed 56 days after MCAo. Immunostaining was performed with antibodies against NG-2 (chondroitin sulfate proteoglycan), CNPase (2", 3"-cyclic nucleotide 3'-phosphodiesterase) to detect immature and mature oligodendrocytes. Neurofilament-H (NF-H) antibodies were used to detect axons while myelinated axons were identified with Bielschowsky/Luxol (B/L) Blue staining. EBA (endothelial barrier antigen) was used for detection of ma...Continue Reading

References

Sep 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·A L GoldsteinA White
Dec 14, 1995·The New England Journal of Medicine·UNKNOWN National Institute of Neurological Disorders and Stroke rt-PA Stroke Study Group
Sep 1, 1996·Stroke; a Journal of Cerebral Circulation·L PantoniJ A Gutierrez
Jul 8, 1996·Neuroreport·N VartiainenJ Koistinaho
Feb 1, 1997·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Z ZhangM Chopp
Mar 29, 2002·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Zheng Gang ZhangMichael Chopp
Jul 3, 2002·Nature Reviews. Neuroscience·M Angela CenciTimothy Schallert
Aug 6, 2002·Nature Medicine·Andreas ArvidssonOlle Lindvall
Sep 27, 2002·Current Eye Research·Gabriel SosneMichelle Kurpakus-Wheater
Nov 2, 2002·Stroke; a Journal of Cerebral Circulation·Ruilan ZhangMichael Chopp
Jun 5, 2003·Annals of Neurology·Jieli ChenMichael Chopp
Apr 17, 2004·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Ruilan ZhangMichael Chopp
Oct 7, 2004·Journal of Cell Science·Thomas HuffHans Georg Mannherz
Feb 25, 2005·Neurology·UNKNOWN California Acute Stroke Pilot Registry (CASPR) Investigators
Aug 16, 2005·Trends in Molecular Medicine·Allan L GoldsteinHynda K Kleinman
Jan 10, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ronald E van KesterenAugust B Smit
Jul 10, 2007·Nature Neuroscience·Adan AguirreVittorio Gallo
Jul 12, 2007·Neurocritical Care·Dawn O KleindorferBrett M Kissela
Jan 25, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Sanja IvkovicJames E Goldman
Oct 22, 2008·Nature Reviews. Neuroscience·Robin J M Franklin, Charles Ffrench-Constant
Oct 3, 2009·Stroke; a Journal of Cerebral Circulation·Dawn KleindorferRichard Hornung

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Citations

Aug 27, 2013·The Protein Journal·Christine AppEwald Hannappel
Oct 11, 2013·Drug Design, Development and Therapy·Tian-Jiao XuMeng Li
Jul 26, 2014·Journal of the Neurological Sciences·D C MorrisM Chopp
Jan 26, 2016·Neurobiology of Disease·Jing ZhangMichael Chopp
Nov 15, 2011·Expert Opinion on Biological Therapy·Allan L GoldsteinHynda K Kleinman
Jan 24, 2015·Expert Opinion on Biological Therapy·Michael Chopp, Zheng Gang Zhang
Sep 18, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Jun-Bin LinQi Wan
Oct 11, 2012·Annals of the New York Academy of Sciences·Daniel C MorrisMichael Chopp
Oct 12, 2012·Annals of the New York Academy of Sciences·Terry TreadwellAllan L Goldstein
Oct 12, 2012·Annals of the New York Academy of Sciences·Ye XiongMichael Chopp
Aug 28, 2012·Neurobiology of Disease·Lei WangZheng Gang Zhang
Mar 26, 2013·Regulatory Peptides·Bo XuDapeng Jiang
Jun 18, 2014·Neuropharmacology·Peng ChengJian Wang
Oct 4, 2011·Experimental Neurology·Xinchun YeJieli Chen
Jul 28, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Giuseppina MaccarroneFrank Weber
Oct 19, 2016·Molecular Neurobiology·Sreekala S NampoothiriRajanikant G Krishnamurthy
Nov 30, 2016·Scientific Reports·Hitomi KurinamiRyuichi Morishita
Aug 1, 2018·Expert Opinion on Biological Therapy·Daniel C MorrisMichael Chopp
Sep 22, 2017·Stem Cells International·Mengchu CuiHua Feng
Apr 16, 2019·Frontiers in Physiology·Kenta Hyeon Tae ChoMhoyra Fraser
Oct 16, 2019·Journal of Cellular Physiology·Navid ShomaliSiamak Sandoghchian Shotorbani

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