Thyroid bud morphogenesis requires CDC42- and SHROOM3-dependent apical constriction

Biology Open
David A F LoebelPatrick P L Tam

Abstract

Early development of the gut endoderm and its subsequent remodeling for the formation of organ buds are accompanied by changes to epithelial cell shape and polarity. Members of the Rho-related family of small GTPases and their interacting proteins play multiple roles in regulating epithelial morphogenesis. In this study we examined the role of Cdc42 in foregut development and organ bud formation. Ablation of Cdc42 in post-gastrulation mouse embryos resulted in a loss of apical-basal cell polarity and columnar epithelial morphology in the ventral pharyngeal endoderm, in conjunction with a loss of apical localization of the known CDC42 effector protein PARD6B. Cell viability but not proliferation in the foregut endoderm was impaired. Outgrowth of the liver, lung and thyroid buds was severely curtailed in Cdc42-deficient embryos. In particular, the thyroid bud epithelium did not display the apical constriction that normally occurs concurrently with the outgrowth of the bud into the underlying mesenchyme. SHROOM3, a protein that interacts with Rho GTPases and promotes apical constriction, was strongly expressed in the thyroid bud and its sub-cellular localization was disrupted in Cdc42-deficient embryos. In Shroom3 gene trap mutant...Continue Reading

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Citations

Jun 22, 2017·Development·Mikael Nilsson, Henrik Fagman
Jul 2, 2021·Genetics in Medicine : Official Journal of the American College of Medical Genetics·Rui-Meng YangHuai-Dong Song

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Methods Mentioned

BETA
GTPases
gene-trap
genotyping

Software Mentioned

Photoshop
SPOT
Pixelmator

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