Thyroid cytology smear slides: An untapped resource for ThyroSeq testing.

Cancer Cytopathology
Marina N NikiforovaZubair Wahid Baloch

Abstract

Molecular testing of thyroid nodules with indeterminate fine-needle aspiration (FNA) cytology is commonly used to guide patient management and is typically performed on freshly collected FNA samples. In this study, the authors evaluated the performance of the ThyroSeq test in cytology smear slides. Air-dried Diff-Quik (DQ)-stained and alcohol-fixed Papanicolaou (Pap)-stained smears were used to determine required cellularity and sensitivity of mutation detection and to compare ThyroSeq v3 Genomic Classifier (GC) results obtained in cytology smears and fresh FNA samples from the same nodules. ThyroSeq testing of 31 cytology smears revealed that 25 smears (81%) were adequate for ThyroSeq analysis, including 14 Pap-stained smears (100%) and 11 DQ-stained smears (65%), whereas 6 DQ-stained smears (35%) failed RNA sequencing. The overall accuracy for detecting molecular alterations was 98%, with 100% concordance for mutations and gene expression alterations, 96% concordance for fusions, and 94% concordance for copy number alterations. Cytology smears were adequate for ThyroSeq analysis when at least 200 to 300 cells were present in 1 to 3 slides. ThyroSeq detected all studied mutations down to 5% allele frequency and BRAF mutations ...Continue Reading

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Citations

Oct 20, 2020·Cancer Cytopathology·Kartik ViswanathanPeter M Sadow
Mar 6, 2021·Current Opinion in Otolaryngology & Head and Neck Surgery·Giorgio GraniCosimo Durante
Jul 19, 2021·Annals of Surgical Oncology·Snehal G PatelAndrew J Lee

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