PMID: 8597565Feb 8, 1996Paper

Thyroid hormone induces a 52 kDa soluble protein in goat testis Leydig cell which stimulates androgen release

Biochimica Et Biophysica Acta
N R JanaS Bhattacharya

Abstract

Incubation of goat testicular Leydig cells with 3,5,3'-triiodothyronine (T3) induces the generation of a proteinaceous factor (factors) which was located in the soluble supernatant fraction (100 000 x g supernatant, 100 k sup) of sonicated Leydig cells. Addition of this factor to Leydig cell incubation greatly stimulated androgen release. This factor(s) was purified based on its biological properties, i.e., its addition to Leydig cell incubation augmented the release of androgen. This was designated as TIP (T3-induced protein) activity. 100 k sup prepared from Leydig cells incubated in the absence (control) or presence of T3 was gel filtered through Sephadex G-100. 100 k sup from T3 incubated gave two protein peaks, P-I and P-II, control 100 k sup had similar nature of P-I, but P-II was not well marked. Incubation in the presence of [14C]leucine clearly showed TCA precipitable radioactivity only in the P-II region of T3 incubate. 5 microg of P-II protein stimulated androgen release from Leydig cells cells (1 x 10(6) cells/well) to more than 5-fold as compared to control. P-II protein was further purifies by FPLC Mono-Q column chromatography where one unadsorbed (MQ-I) and two adsorbed (MQ-II and MQ-III) protein peaks could be d...Continue Reading

References

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Citations

Jan 6, 2000·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·R J Nagendra PrasadS Bhattacharya
Jun 1, 2001·The Journal of Steroid Biochemistry and Molecular Biology·P R MannaI T Huhtaniemi
Aug 1, 2007·Animal : an International Journal of Animal Bioscience·L Todini
Dec 17, 2009·Acta Veterinaria Scandinavica·David GunnarssonAndrzej Madej
Aug 30, 2008·The Journal of Endocrinology·Márcia Santos WagnerAna Luiza Maia
Apr 5, 2014·Indian Journal of Endocrinology and Metabolism·Anand KumarBodhana Dhole
Aug 2, 2003·Archives of Andrology·R R M Maran
Nov 22, 2011·Experimental Biology and Medicine·Mohammed Z Allouh

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